A260 of a DNA was found to be high. It wasnot affected by an increase in temperature.Why?renaturing dna by allowing strands tohybridize is also called——•he amino acids which are dominant inproteins that bind to dna arethe nucleoside that is composed of deoxyribose linked to a guanine

The Absorbance of DNA is observed to show a broad peak at 260 nm. The absorbed double of stranded…

Answered: A260 of a DNA was found to be high. It…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Determine the melting point for the three DNA samples and list two factors that could affect the DNA sample
Which of the following molecules is a nitrogen base that would be found in 1 po DNA? (check all that apply) * Adenine Guanine Uracil Baycil Thymine Qualine Cytosine Lenovo esc -> @ # 2 7 8. tab W e r y u i d f g. k a S 31 C V alt alt ctri .... V -
You turn up the heat to 95° Which is the most likely outcome? Explain your answer. Both duplexes are now single-stranded oligonucleotides Both duplexes are now individual dNTPs Both duplexes remain duplexes The nitrogenous bases are released from the 1’ C of deoxyribose
. Which of the following equations is a prediction based on Chargaff’s rules for the content of DNA? adenine + thymine = guanine + cytosine thymine + adenine = cytosine + guanine guanine + cytosine = adenine + thymine cytosine + guanine = thymine + adenine adenine + cytosine = thymine + guanine
Total nucleic acids are extracted from a growing culture of yeast cells. They are then mixed with specialized beads to which the single-stranded DNA molecule with sequence 5’-TTTTTTTTTTTTTTTTTTTTTTTTT-3’ has been covalently attached to the surface (see image to the right, where each black line represents a polynucleotide sequence). After a short incubation time, the beads are removed from the mixture. When you analyze the cellular nucleic acids stuck to the beads, which type of nucleic acid (i.e. DNA, rRNA, etc.) do you expect to be the most abundant? Why?
a) It is known that double stranded DNA is denatured at low pH. pKa values should allow the determination of whether this is due to perturbation of the hydrogen bonding in A-T and/or G-C base pairs. The table gives values for the pKas of different protonated groups in the nucleobases.Nucleobase Position & pKa A N1, 3.5 G N7, 1.6; N1, 9.2 C N3, 4.2 T N3, 9.7a) Draw the A-T and G-C base pairs. - Label the bases with the one-letter code. – - Number the atoms in the rings and label the atom that attaches to the sugar. - Mark the groups that interact in normal…
What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.
Draw out the molecular structure of a double stranded DNA molecule. This molecule is 6 bp in size. Don't forget label.
It is known that double stranded DNA is denatured at low pH. pKa values should allow the determination of whether this is due to perturbation of the hydrogen bonding in A-T and/or G-C base pairs. The table gives values for the pKas of different protonated groups in the nucleobases.Nucleobase Position & pKa A N1, 3.5 G N7, 1.6; N1, 9.2 C N3, 4.2 T N3, 9.7a) Draw the A-T and G-C base pairs. - Label the bases with the one-letter code. – - Number the atoms in the rings and label the atom that attaches to the sugar. - Mark the groups that interact in normal…
Select all that apply
One strand of DNA has the base sequence 5'-CATG-3' Draw this stretch of DNA, showing BOTH strands, using a simplified model of a nucleotide as shown: Label the sugar-phosphate backbone, the 5' and 3' ends of each strand, and the regions of hydrogen bonding.
What is the purpose of using the Chelex beads while extracting the DNA at high temperature from your cheek cells? Group of answer choices The Chelex binds cations like Mg2+ that are co-factors for nuclease enzymes which would otherwise degrade the DNA. The Chelex denatures the DNA and makes the DNA single stranded. The Chelex will bind the DNA tightly after the cells are ruptured, helping extract it from the cells. The Chelex provides Mg2+ co-factors needed by DNA polymerase enzymes to make more copies of DNA

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A260 of a DNA was found to be high. It was not affected by an increase in temperature. Why? renaturing dna by allowing strands to hybridize is also called ——• he amino acids which are dominant in proteins that bind to dna are the nucleoside that is composed of deoxy ribose linked to a guanine