a. Calculate the retention factor for each spot from lanes A to E . b. Which ink standard was the most polar? least polar? c. What is the composition of ink D ?

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2. Different marker inks were analyzed using a simple paper chromatography
experiment. Two marker inks were dissolved separately in a solvent and spotted
in the chromatogram. The mobile phase used was 1% NaCl and three ink
standards (A-copper-phthalocyanine blue, B-diarylide yellow,C- lithol rubine red)
were spotted beside the two sample markers. After the chromatography, the
chromatogram showed a separation of colors in the two inks (D and E).
a. Calculate the retention factor for each spot from lanes A to E .
b. Which ink standard was the most polar? least polar?
c. What is the composition of ink D ?

uler is in
munit
B
с
BEFORE
D
E
A
B
с
AFTER
E
Transcribed Image Text:uler is in munit B с BEFORE D E A B с AFTER E
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