A two mL stock of Marburg virus (Filamentous, non-segmented, negative strand RNA virusin Filoviridae family) contains 5.6 x 1017 virions (individual viruses)/mL. The sample mustbe diluted serially to the point in which 2.4 x 106 virions exist in every 100 µL of sample foran infection experiment which requires at least 7.2 mL (you will NEED more than 6 mL).Using dilutions of no greater than 1/1000 in volumes no greater than 10 mL and includingone dilution of 1/8.8 show below how you would set up this dilution series. Remember smallvolumes carry inherently larger error.

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Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.