A stock solution whose concentration is 25.00 mg/mL was provided. A primary standard solution was then prepared by transferring 5.00 mL of the stock solution into a 50.00 mL volumetric flask. The primary standard solution was used to prepare three standard solutions of different concentrations by taking different volumes of aliquots into three 25.00 mL volumetric flasks: Standard Solution #1 #2 #3 Amount of Primary Solution (mL) 5.00 10.00 15.00 Three 25.00 mL volumetric flasks were then diluted to mark with water. Questions #1 What is the concentration of the primary standard solutions, expressed in units of mg/ml? #2 Re-write the concentration of the primary standard solutions obtained from Q#1 in unit of μg/mL. #3 Determine the concentrations of three standard solutions in units of µg/mL.

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A stock solution whose concentration is 25.00 mg/mL was provided. A primary standard
solution was then prepared by transferring 5.00 mL of the stock solution into a 50.00 mL
volumetric flask. The primary standard solution was used to prepare three standard
solutions of different concentrations by taking different volumes of aliquots into three 25.00
mL volumetric flasks:
Standard Solution
#1
#2
#3
Amount of Primary Solution
(mL)
5.00
10.00
15.00
Three 25.00 mL volumetric flasks were then diluted to mark with water.
Questions
#1 What is the concentration of the primary standard solutions, expressed in units of
mg/ml?
#2
Re-write the concentration of the primary standard solutions obtained from Q#1 in units
of μg/mL.
#3 Determine the concentrations of three standard solutions in units of µg/mL.
#4 What is a requirement for a "blank" solution used in calibration in spectrophotometry?
Page 5 (Experiment #3)
Transcribed Image Text:A stock solution whose concentration is 25.00 mg/mL was provided. A primary standard solution was then prepared by transferring 5.00 mL of the stock solution into a 50.00 mL volumetric flask. The primary standard solution was used to prepare three standard solutions of different concentrations by taking different volumes of aliquots into three 25.00 mL volumetric flasks: Standard Solution #1 #2 #3 Amount of Primary Solution (mL) 5.00 10.00 15.00 Three 25.00 mL volumetric flasks were then diluted to mark with water. Questions #1 What is the concentration of the primary standard solutions, expressed in units of mg/ml? #2 Re-write the concentration of the primary standard solutions obtained from Q#1 in units of μg/mL. #3 Determine the concentrations of three standard solutions in units of µg/mL. #4 What is a requirement for a "blank" solution used in calibration in spectrophotometry? Page 5 (Experiment #3)
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