A stock solution of alcohol dehydrogenase (ADH) has been diluted 1 in 10 and the absorbance reading at 280 nm of this diluted solution is 0.04. Given that the absorption coefficient for ADH at 280 nm is e mg/ml = 1.6 and that you require to do 30 assays in triplicate, each assay using 2 ug of enzyme, how many ul of the stock ADH do you need to do the experiment?

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.