A standard 3 mL cuvette is two-thirds filled with a 1 in 5 dilution of 70 mM oxyhaemoglobin. The absorbance is measured at 580nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.40, a decrease of 0.2 on the first measurement. What is the molar absorbance coefficient of the oxyhaemoglobin at 580 nm? (do not enter the units in the answer, 2 decimal places)
A standard 3 mL cuvette is two-thirds filled with a 1 in 5 dilution of 70 mM oxyhaemoglobin. The absorbance is measured at 580nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.40, a decrease of 0.2 on the first measurement. What is the molar absorbance coefficient of the oxyhaemoglobin at 580 nm? (do not enter the units in the answer, 2 decimal places)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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A standard 3 mL cuvette is two-thirds filled with a 1 in 5 dilution of 70 mM oxyhaemoglobin. The absorbance is measured at 580nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.40, a decrease of 0.2 on the first measurement. What is the molar absorbance coefficient of the oxyhaemoglobin at 580 nm? (do not enter the units in the answer, 2 decimal places)
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