A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0 227 0.148 650 nm 0230 0230 700 nm 0.151 0.357 750 nm 0.157 0246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. Amax Jerry STD CURVE JERRY STD CURVE BEN Amax Ben Amax Ben Amаx Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0 284 800 0.607 0218 S00 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457 Using the given data, determine the following information: What is the Amax of Ben? O 750 nm O 400 nm O 450 nm O 650 nm İ..

A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0 227 0.148 650 nm 0230 0230 700 nm 0.151 0.357 750 nm 0.157 0246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. Amax Jerry STD CURVE JERRY STD CURVE BEN Amax Ben Amax Ben Amаx Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0 284 800 0.607 0218 S00 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457 Using the given data, determine the following information: What is the Amax of Ben? O 750 nm O 400 nm O 450 nm O 650 nm İ..

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question 23 Using the given data above, determine the following information: What is the Amax of Jerry? 650 nm O 500 nm O 700 nm O 800 nm