What is the linearity (r2) value of the following concentration and absorbance values: SAMPLE CONCENTRATION ABSORBANCE 1 0.178 0.2127 2 0.274 0.2715 3 0.473 0.3892 0.574 0.4568 0.673 0.5327 6 0.864 0.6283 0.9876 0.9786 O 0.9562 O 0.9976
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- What is the linearity (r2) value of the following concentration and absorbance values: SAMPLE CONCENTRATION ABSORBANCE 1 0.178 0.2127 2 0.274 0.2715 3 0.473 0.3892 4 0.574 0.4568 5 0.673 0.5327 6 0.864 0.6283 0.9786 0.9876 0.9562 0.9976Unrounded Rounded ε∗,L/μmol 0.0259825 0.0260 Heres the data if needed: TZ # Concentration Absorbance at 430 nmnm 1 33.6480 0.931 2 25.2360 0.757 3 16.8420 0.210 4 8.41200 0.137 5 4.20600 0.122heres the data TZ # Concentration Absorbance at 430 nmnm 1 33.6480 0.931 2 25.2360 0.757 3 16.8420 0.210 4 8.41200 0.137 5 4.20600 0.122
- Given the following sample concentrations and corresponding absorbances, construct a standard curve of absorbance vs. concentration and determine the molar absorptivity constant. Sample Concentration 15 μΜ 25 μΜ 40 μΜ Absorbance 0.19 0.32 0.49 55 μΜ 0.70Which data point in your standard curve is the most important in determining the best straight line 1. through your data? Why? Which is the second most important? Why? pathlength cell displays % T of 50.0 what is the Absorbance of the solution and what is the absorptivity (in /cm-mole). What Absorbance would be expected for a 5.0 x 10-6 M solution? What % T would this correspond to? 2. A solution of a light absorbing species with a concentration of 1.0 x 10-5M in a 1.0 cmWhat is the extinction coefficient for the following absorbances? 1. 3.000 2. 2.022 3.1.2025 4. 0.824 Would the units be in µmoles/mL)^-1 cm^-1
- 3. If you wished to study the molecule below by measuring absorbance, what wavelength should you set your spectrophotometer to? 1.6 Absorbance 1.4 1.2 1.0 0.8 0.6 0.4- 0.2- ME 0.0 300 400 600 Wavelength (nm) 500 Solutions and Mol....pdf 2 700 20221027_141317.jpg 800Briefly explain why it is NOT accurate to calculate the concentration of a sample whose absorbance is 0.185 using the equation y=0.0022x+0.0005 r^2=0.999A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…
- A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…Spectral Analysis IR Analysis Interpret the IR spectrum. Transmitance 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 3500 3000 Infrared Spectrum 2500 Wavenumbers (cm-1) 2000 Cha 1500 1000