A sample of chromatin was partially digested by the enzyme staphy- lococcal nuclease. The DNA fragments from this digestion were purified and resolved on a polyacrylamide gel. A set of DNA restric- tion fragments was used as markers. Distances of migration are given below. From these data, estimate the nucleosome repeat dis- tance in the chromatin. Marker DNA Fragment Chromatin DNA Fragment Size (bp) d (cm) d (cm) 94 40 30.5 145 34.2 19.2 263 25.2 14.4 498 16.7 11.5 794 11.5

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A sample of chromatin was partially digested by the enzyme staphy-
lococcal nuclease. The DNA fragments from this digestion were
purified and resolved on a polyacrylamide gel. A set of DNA restric-
tion fragments was used as markers. Distances of migration are
given below. From these data, estimate the nucleosome repeat dis-
tance in the chromatin.
Marker DNA Fragment
Chromatin DNA Fragment
Size (bp)
d (cm)
d (cm)
94
40
30.5
145
34.2
19.2
263
25.2
14.4
498
16.7
11.5
794
11.5
Transcribed Image Text:A sample of chromatin was partially digested by the enzyme staphy- lococcal nuclease. The DNA fragments from this digestion were purified and resolved on a polyacrylamide gel. A set of DNA restric- tion fragments was used as markers. Distances of migration are given below. From these data, estimate the nucleosome repeat dis- tance in the chromatin. Marker DNA Fragment Chromatin DNA Fragment Size (bp) d (cm) d (cm) 94 40 30.5 145 34.2 19.2 263 25.2 14.4 498 16.7 11.5 794 11.5
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