"A protein has a pI point which can be used to separate proteins using ion exchange. If you have an anion exchange column and access to buffers of any pH, how could you isolate a protein with a pI of 7 from a mixture of proteins which also has proteins with pI points of 3 and 5" Write a few sentences or draw a picture.

Q: Draw the structure of the most prominent form of tyrosine at pH 1.9

A: We have to draw the structure tyrosine at pH 1.9

Q: All amino acids have two ionizable functional groups: an α‑amino group (average p?a of 9.4) and an…

A: The ionization of Aspartic acid according to the question is shown in the figure:

Q: Which would be the best choice as a buffer for an enzyme reaction with a pH optimum of 7.4?…

A: Buffer solution: The solution that resists the change in pH is known as a buffer solution. The…

Q: Answer the following questions on the basis of the free energies of formation at 25°C shown in the…

A: (a) The mentioned peptide bond formation reaction can be represented as:…

Q: Qualitative Test for Proteins

A:

Q: Consider the hypothetical serine protease in the image, which shows the specificity pockets. The S,…

A: Hydrophobic side chains are non-polar side chains. The hydrophilic side chains are polar, acidic,…

Q: In a typical laboratory set-up, what conditions are necessary in carrying out acid/base hydrolysis…

A: In typical laboratory set-up we have to be more careful while carrying out acid/base hydrolysis of…

Q: How does adding a base allow us to let us know that proteins are in the sample that we're trying to…

A: On adding Acid and base in the sample contains protein, the pH of the sample as well as protein get…

Q: Arginine has ionizable groups with pKa values of 2.17, 9.04, and 12.48. A researcher makes up 73 mL…

A: From the pKa values of arginine, it can be stated that, at pH 8.5 second proton is partially…

Q: You run a DEAE-cellulose column to purify anions from a biological sample. Which approach, from the…

A: We are running the column chromatography of DEAE-cellulose to separate anions. We are trying here to…

Q: 7*) Knowing that there are 20 different possible amino acids that can be used at each position in a…

A: According to the question, single amino acid can be used many times. There are 20 amino acids and…

Q: What is/are the product/s of this reaction?

A: Given,

Q: Why would a protein likely remain soluble at pH conditions either above or below the protein’s pI,…

A: The solution is given below -

Q: Select the correct statements about the isoelectric point of a protein. ( all that may applied ) -…

A: Isoelectric point is the pH at which the substance is electrically neutral.

Q: All amino acids have two ionizable functional groups: an α‑amino group (average pKa of 9.4) and an…

A: The question is based on the concept of amino acids. we have to identify at which pH, this structure…

Q: how could the formulation scientist prevent aggregation of a protein drug?

A: Protein aggregation: A biological phenomenon in which intrinsically misfolded proteins or disordered…

Q: what is the role of Cu+2 ion in biuret test for proteins?

A: Biuret test is used to detect the presence of peptide bonds in a molecule.

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.