A Mouse brain kDa Control Atg7 CKO 250- 50- 50- 50- 37- E MEF with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 50- 50- 15- 37- F Relative protein level +AKAP11 + Rla 1.5* Rila 1.0 0.5- 0.0+ 0 3 6 Atg7 WT -AKAP11 -Rila -Rla -p62 -Actin gh Relative protein level 1.5 1.0 0.5- B Control Atg7 CKO 8₁ 0.0 Relative Protein level -AKAP11 -Rila -Rla -LC31 -LC311 -Actin + ल 2 AKAP11 +AKAP11 +Rla Rila J Ria ns Rila с kDa 250- 50- 50- 50- 37- G 50- 50- HEK293T with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 15- 37- H Relative protein level Mouse brain Control Atg14 CKO 1.0 +Rla 1.5* Rila 0.5- +AKAP11 0.0+ # # 6 9h -AKAP11 -Rila -Ria -p62 -Actin 3 Atg7 WT Relative protein level 1.0 Relative Protein level 0.5- 8- 0.0+ 9.00 99 -AKAP11 -Rila -Ria LLC31 FLC311 -Actin 2 1.5 Rila *² Control Atg14 CKO AKAP11 AKAP11 - Rla J Rla 3 6 gh Atg7 KO Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM (n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre- sented as mean ± SEM (n = 4 mice/genotype). **P<0.01; ***P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean + SEM (n = 3). *P<0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean ± SEM (n = 4). "P < 0.05; **P < 0.01; ***P < 0.001. 3 6 9h Atg7 KO ns Rila CELL BIOLOGY

A Mouse brain kDa Control Atg7 CKO 250- 50- 50- 50- 37- E MEF with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 50- 50- 15- 37- F Relative protein level +AKAP11 + Rla 1.5* Rila 1.0 0.5- 0.0+ 0 3 6 Atg7 WT -AKAP11 -Rila -Rla -p62 -Actin gh Relative protein level 1.5 1.0 0.5- B Control Atg7 CKO 8₁ 0.0 Relative Protein level -AKAP11 -Rila -Rla -LC31 -LC311 -Actin + ल 2 AKAP11 +AKAP11 +Rla Rila J Ria ns Rila с kDa 250- 50- 50- 50- 37- G 50- 50- HEK293T with starvation Atg7 WT Atg7 KO kDa 0 3 6 9 0 3 6 9h 250- 15- 37- H Relative protein level Mouse brain Control Atg14 CKO 1.0 +Rla 1.5* Rila 0.5- +AKAP11 0.0+ # # 6 9h -AKAP11 -Rila -Ria -p62 -Actin 3 Atg7 WT Relative protein level 1.0 Relative Protein level 0.5- 8- 0.0+ 9.00 99 -AKAP11 -Rila -Ria LLC31 FLC311 -Actin 2 1.5 Rila ² Control Atg14 CKO AKAP11 AKAP11 - Rla J Rla 3 6 gh Atg7 KO Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM (n = 3 mice/genotype). P < 0.01; P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre- sented as mean ± SEM (n = 4 mice/genotype). P<0.01; P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean + SEM (n = 3). P<0.05; P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and values are presented as mean ± SEM (n = 4). "P < 0.05; P < 0.01; ***P < 0.001. 3 6 9h Atg7 KO ns Rila CELL BIOLOGY

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Fig. 1. AKAP11 and RIα are degraded by autophagy. (A) Brain lysates of Atg7F/F (Control) and Atg7F/F-SynCre (cKO) were subjected to immunoblotting
analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student’s t tests were used, and values are presented as mean ± SEM
(n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F/F (Control) and Atg14F/F-SynCre (cKO) were subjected to
immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student’s t tests were used, and values are presented as mean ± SEM (n = 4 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using
EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E
was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype,
and values are presented as mean ± SEM (n = 3). *P < 0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the
indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was
obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and
values are presented as mean ± SEM (n = 4). #
P < 0.05; **P < 0.01; ***P < 0.001.

explain each diagram from A-H please!

A
Mouse brain
kDa Control Atg7 CKO
250-
50-
50-
50-
37-
E
MEF with starvation
Atg7 WT
Atg7 KO
kDa 0 3 6 9 0 3 6 9h
250-
50-
50-
15-
37-
F
Relative protein level
+AKAP11
+ Rla
1.5* Rila
1.0
0.5-
0.0+
0
3 6
Atg7 WT
-AKAP11
-Rila
-Rla
-p62
-Actin
gh
Relative protein level
1.5
1.0
0.5-
B Control
Atg7 CKO
8₁
0.0
Relative Protein level
-AKAP11
-Rila
-Rla
-LC31
-LC311
-Actin
+
ल
2
AKAP11
+AKAP11
+Rla
Rila
J
Ria
ns
Rila
с
kDa
250-
50-
50-
50-
37-
G
50-
50-
HEK293T with starvation
Atg7 WT Atg7 KO
kDa 0 3 6 9 0 3 6 9h
250-
15-
37-
H
Relative protein level
Mouse brain
Control Atg14 CKO
1.0
+Rla
1.5* Rila
0.5-
+AKAP11
0.0+
#
#
6 9h
-AKAP11
-Rila
-Ria
-p62
-Actin
3
Atg7 WT
Relative protein level
1.0
Relative Protein level
0.5-
8-
0.0+
9.00
99
-AKAP11
-Rila
-Ria
LLC31
FLC311
-Actin
2
1.5 Rila
*²
Control
Atg14 CKO
AKAP11
AKAP11
- Rla
J
Rla
3 6 gh
Atg7 KO
Fig. 1. AKAP11 and Rla are degraded by autophagy. (A) Brain lysates of Atg7FF (Control) and Atg7fF-SynCre (CKO) were subjected to immunoblotting
analysis with the indicated antibodies. (B) Quantification of protein levels in A. Unpaired Student's t tests were used, and values are presented as mean + SEM
(n = 3 mice/genotype). **P < 0.01; ***P < 0.001; ns, not significant. (C) Brain lysates of Atg14F (Control) and Atg14FF-SynCre (cKO) were subjected to
immunoblotting analysis with the indicated antibodies. (D) Quantification of protein levels in C. Unpaired Student's t tests were used, and values are pre-
sented as mean ± SEM (n = 4 mice/genotype). **P<0.01; ***P<0.001; ns, not significant. (E) Atg7 WT and Atg7 KO MEF cells were nutrient starved by using
EBSS for the indicated times, and cells were assayed by immunoblotting analysis with the indicated antibodies. (F) Quantification of the protein levels from E
was obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype,
and values are presented as mean + SEM (n = 3). *P<0.05; **P < 0.01. (G) Atg7 WT and Atg7 KO HEK293T cells were nutrient starved by using EBSS for the
indicated times and were then assayed by immunoblotting analysis with the indicated antibodies. (H) Quantification of the protein levels from G was
obtained by normalizing the level of proteins to Actin, respectively, and further to the respective control. One-way ANOVA was used in each genotype, and
values are presented as mean ± SEM (n = 4). "P < 0.05; **P < 0.01; ***P < 0.001.
3 6 9h
Atg7 KO
ns
Rila
CELL BIOLOGY

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