O The hybridomas will be selected but theycan't be grown on a large scale in the futurewith inexpensive sugar and amino acids inthe media. The selected hybridomas willalways need a supplemented media withhypoxanthine and thymidine.The hybridomas will be selected and thereshould be no problem afterward either.O The unfused myeloma cells and thehybridoma cells both will grow togetherindefinitely in the absence of aminopterin inthe media and the selection of thehybridoma cells will not be possibleO The experiment will fail and no hybridomaselection will take place. You are in the process of developing amonoclonal antibody against an Influenzavirus spike protein "H7."You have isolated the B cells producingantibodies against the viral H7 protein andfused those specific B cells with a uniquekind of mutant myeloma cells. Themyeloma cells can't make any of thethree following enzymes; dihydrofolatereductase (DHFR), thymidine kinase (TK),and hypoxanthine-guanosinephosphoribosyl transferase (HGPRT). Themutant unfused myeloma cells are grownin the laboratory in a nucleotidesupplemented medium.For selecting the hybridomas, you haveused a modified medium HT that does notcontain any aminopterin but containshypoxanthine and thymidine.Which of the following result do youexpect to see from your experiment, andwhat problem you may encounter in thefuture after the hybridoma selection?

Answered: Which of the following result do you…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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O The hybridomas will be selected but they
can't be grown on a large scale in the future
with inexpensive sugar and amino acids in
the media. The selected hybridomas will
always need a supplemented media with
hypoxanthine and thymidine.
The hybridomas will be selected and there
should be no problem afterward either.
O The unfused myeloma cells and the
hybridoma cells both will grow together
indefinitely in the absence of aminopterin in
the media and the selection of the
hybridoma cells will not be possible
O The experiment will fail and no hybridoma
selection will take place.

You are in the process of developing a
monoclonal antibody against an Influenza
virus spike protein "H7."
You have isolated the B cells producing
antibodies against the viral H7 protein and
fused those specific B cells with a unique
kind of mutant myeloma cells. The
myeloma cells can't make any of the
three following enzymes; dihydrofolate
reductase (DHFR), thymidine kinase (TK),
and hypoxanthine-guanosine
phosphoribosyl transferase (HGPRT). The
mutant unfused myeloma cells are grown
in the laboratory in a nucleotide
supplemented medium.
For selecting the hybridomas, you have
used a modified medium HT that does not
contain any aminopterin but contains
hypoxanthine and thymidine.
Which of the following result do you
expect to see from your experiment, and
what problem you may encounter in the
future after the hybridoma selection?

Expert Solution

Step 1: B-cells

Immunity is the term used for the ability of our body to fight against pathogens. Immunity is of two types: innate and adaptive immunity. B-cells are part of adaptive immunity. They are a type of white blood cell (WBC). They produce specific proteins that are used to kill pathogens, which are called antibodies. There are two types of B-cells: plasma cells and memory cells. Plasma cells are mature B-cells that can release antibodies in response to antigens. Memory B-cells are produced after encountering an antigen, They are used to remember particular antigens and help in the quick response when that particular antigen is encountered again.

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