A) Immunoprecipitation and immunoblotting. Sarcoma cell protein extracts were incubated with antibodies specific for V. The antibodies to V, and the proteins bound to them, were collected and subjected to polyacrylamide gel electrophoresis (PAGE; a technique to separate proteins based on size). The separated proteins were transferred from the PAGE gels onto a nylon membranes. Lastly, the membranes were incubated with labeled antibodies to Cdk2, Cdk4, and/or Cdk6 (right panel) or with labeled antibodies to cyclin D1 (center panel). As a control, the Sarcoma cell protein extracts were subjected to PAGE, transferred to membranes, and incubated with labeled antibodies to Cdk2, Cdk4, and/or Cdk6 (left panel). cyclin D1 total Prot.V lysate Cdk2 1. What is the purpose of the control shown in the left panel? Cdk4 2. What do the results in the right panel demonstrate? Protein V might activate Cdk4/6 with the expression of cyclin D1 Cdk6

A) Immunoprecipitation and immunoblotting. Sarcoma cell protein extracts were incubated with antibodies specific for V. The antibodies to V, and the proteins bound to them, were collected and subjected to polyacrylamide gel electrophoresis (PAGE; a technique to separate proteins based on size). The separated proteins were transferred from the PAGE gels onto a nylon membranes. Lastly, the membranes were incubated with labeled antibodies to Cdk2, Cdk4, and/or Cdk6 (right panel) or with labeled antibodies to cyclin D1 (center panel). As a control, the Sarcoma cell protein extracts were subjected to PAGE, transferred to membranes, and incubated with labeled antibodies to Cdk2, Cdk4, and/or Cdk6 (left panel). cyclin D1 total Prot.V lysate Cdk2 1. What is the purpose of the control shown in the left panel? Cdk4 2. What do the results in the right panel demonstrate? Protein V might activate Cdk4/6 with the expression of cyclin D1 Cdk6

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter16: Antifungal, Antiviral, And Immunizing Agents

Section: Chapter Questions

Problem 25RQ

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The excess accumulation of pollutants or undesirable alterations in the environment and its resources such as air, water, and land can be referred to as pollution. Pollution affects the components of the environment. Pollution is considered a global issue as it is showing harmful effects worldwide. Metropolitan cities are more impacted by pollution than countryside or villages.

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(Cdk). They performed the experiments below to test their hypothesis.
A) Immunoprecipitation and immunoblotting. Sarcoma cell protein extracts were incubated
with antibodies specific for V. The antibodies to V, and the proteins bound to them,
were collected and subjected to polyacrylamide gel electrophoresis (PAGE; a technique
to separate proteins based on size). The separated proteins were transferred from the
PAGE gels onto a nylon membranes. Lastly, the membranes were incubated with
labeled antibodies to Cdk2, Cdk4, and/or Cdk6 (right panel) or with labeled antibodies to
cyclin D1 (center panel). As a control, the Sarcoma cell protein extracts were subjected
to PAGE, transferred to membranes, and incubated with labeled antibodies to Cdk2,
Cdk4, and/or Cdk6 (left panel).
cycdin
D1
total
Prot.V
lysate
Cdk2
1. What is the purpose of the control shown in the left panel?
Cdk4
2. What do the results in the right panel demonstrate?
Protein V might activate Cdk4/6 with the expression of cyclin
Cdk6
D1
B) Enzyme assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks
nhognhorurloto retinohlastima pro
•in (RB). Purified Y was incubated with purified

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