A donor strain of bacteria with alleles a+ b+ c+ is infected with phages to map the donor chromosome using generalized transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a− b− c−. Bacteria with the a+ allele are selected, and the percentage of cells with cotransduced b+ and c+ alleles are recorded. Donor Recipient Selected allele Cells with cotransduced allele (%) a+ b+ c+ a− b− c− a+ 25 b+ a+ 3 c+ Is gene b or gene c closer to gene a? Explain your reasoning.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
A donor strain of bacteria with alleles a+ b+ c+ is infected with
phages to map the donor chromosome using generalized
transduction. The phage lysate from the bacterial cells is collected
and used to infect a second strain of bacteria that are a− b− c−.
Bacteria with the a+ allele are selected, and the percentage of cells
with cotransduced b+ and c+ alleles are recorded.
Donor Recipient Selected
allele
Cells with cotransduced
allele (%)
a+ b+
c+ a− b− c− a+ 25 b+
a+ 3 c+
Is gene b or gene c closer to gene a? Explain your reasoning.
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