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A biologically active protein produced in Escherichia coli fermentations is purified byprecipitation. A 200 L solution containing 5.2 wt% protein is treated in a batch precipitationreactor. 1 kg of salt is added to induce protein precipitation. At the end of the process, theremaining solution contains 1.5 wt% protein, and the precipitate is wet with 5wt% of water.(a) What is the mass of the residual protein solution?(b) What mass of protein precipitate is produced?Assume the density of the solution remains approximately 1.00 g/mL throughout the process,and that the concentration of salt in the water captured in the precipitate is the same as theconcentration of salt in total.

A biologically active protein produced in Escherichia coli fermentations is purified byprecipitation. A 200 L solution containing 5.2 wt% protein is treated in a batch precipitationreactor. 1 kg of salt is added to induce protein precipitation. At the end of the process, theremaining solution contains 1.5 wt% protein, and the precipitate is wet with 5wt% of water.(a) What is the mass of the residual protein solution?(b) What mass of protein precipitate is produced?Assume the density of the solution remains approximately 1.00 g/mL throughout the process,and that the concentration of salt in the water captured in the precipitate is the same as theconcentration of salt in total.

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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A biologically active protein produced in Escherichia coli fermentations is purified by
precipitation. A 200 L solution containing 5.2 wt% protein is treated in a batch precipitation
reactor. 1 kg of salt is added to induce protein precipitation. At the end of the process, the
remaining solution contains 1.5 wt% protein, and the precipitate is wet with 5wt% of water.
(a) What is the mass of the residual protein solution?
(b) What mass of protein precipitate is produced?
Assume the density of the solution remains approximately 1.00 g/mL throughout the process,
and that the concentration of salt in the water captured in the precipitate is the same as the
concentration of salt in total. 

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