A bacterial operon is responsible for the production of the biosyntheticenzymes needed to make the hypothetical amino acidtisophane (tis). The operon is regulated by a separate gene, R.The deletion of R causes the loss of enzyme synthesis. In the wildtypecondition, when tis is present, no enzymes are made; in theabsence of tis, the enzymes are made. Mutations in the operatorgene (O-) result in repression regardless of the presence of tis. Isthe operon under positive or negative control? Propose a modelfor (a) repression of the genes in the presence of tis in wild-typecells and (b) the mutations.
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A bacterial operon is responsible for the production of the biosynthetic
enzymes needed to make the hypothetical amino acid
tisophane (tis). The operon is regulated by a separate gene, R.
The deletion of R causes the loss of enzyme synthesis. In the wildtype
condition, when tis is present, no enzymes are made; in the
absence of tis, the enzymes are made. Mutations in the operator
gene (O-) result in repression regardless of the presence of tis. Is
the operon under positive or negative control? Propose a model
for (a) repression of the genes in the presence of tis in wild-type
cells and (b) the mutations.
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