A 1L buffer solution needs to be prepared. The buffer will be composed of the following, 200mM tris, 400mM glycine, and 0.5% (w/v) glycerol. The stock reagents for tris (124.14g/mol) and glycine (75.07g/mol) are stored as separate lyophilized powders. Glycerol is stored as a liquid at 20% (w/v). Approximately how many mL of glycerol should be measured for this buffer?
A 1L buffer solution needs to be prepared. The buffer will be composed of the following, 200mM tris, 400mM glycine, and 0.5% (w/v) glycerol. The stock reagents for tris (124.14g/mol) and glycine (75.07g/mol) are stored as separate lyophilized powders. Glycerol is stored as a liquid at 20% (w/v). Approximately how many mL of glycerol should be measured for this buffer?
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A 1L buffer solution needs to be prepared. The buffer will be composed of the following, 200mM tris, 400mM glycine, and 0.5% (w/v) glycerol. The stock reagents for tris (124.14g/mol) and glycine (75.07g/mol) are stored as separate lyophilized powders. Glycerol is stored as a liquid at 20% (w/v). Approximately how many mL of glycerol should be measured for this buffer?
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Given: buffer with composition 200mM tris, 400mM glycine, and 0.5% (w/v) glycerol.
To find: volume of glycerol to be measured for this buffer in mL.
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