A 0.00000001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 150 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single ml of the original culture? Express your answer to two decimal places using exponential notation. • Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
A 0.00000001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 150 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single ml of the original culture? Express your answer to two decimal places using exponential notation. • Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:A 0.00000001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution,
*0.1 mL* of solution is plated on solid media, and 150 colonies of bacteria grow on the petri dish.
Assuming each colony came from a single bacterium, how many bacteria are in a single ml of the original culture?
Express your answer to two decimal places using exponential notation.
• Since only 0.1 mL is put on the plate, this counts as an extra dilution!!!
Any time less than 1 mL is transfered, a dilution is being performed.
Any time more than 1 mL is transfered, a concentration is being performed.
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