9.) Riboflavin is an interesting B-vitamin (Vitamin B2) with the givenstructure. Could you use the following techniques to detectriboflavin? For each answer give a short sentence why or why not:CHNH.CH 3OHA.) FluorescenceHOHOB.) UV-Vis absorbanceC.) GC-FIDHO.D.) ESI (with quadrupole)E.) PotentiometryF.) ICP-MSG.) IRH.) SIMS mass spectrometry1.) AA

Riboflavin/ Vitamin B2 is a dietary supplement which is consumed through food. It is required for…

Answered: 9.) Riboflavin is an interesting…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Which of the following linkages is found in sphingolipids? a. sphingosine-choline-phosphate b. sphingosine-phosphate-choline c. phosphate-sphingosine-choline d. none of the above
20 4. A sample containing ethanol and water was found to have n6 = 1.3400. Calculate the exact percentage of ethanol and water in this example. Show your work. (Hint: Solve this mathematically. Assume a linear relationship between concentration and refractive index. You can make the y-intercept the refractive index of water, and use the slope formula to find the slope. The x axis (concentration) will be from 0% ethanol to 100% ethanol. From this information, you can get the equation of the line, then plug in your numbers from there.)
Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM.
25. Assume we are performing a 2x serial dilution series of methylene similar to WL-1. If your original, 1x tube is designated "Tube 1", then what would be the relative concentration of methylene blue in Tube 4? a. Tube 4 is the 8x dilution tube. The concentration of methylene blue in this tube is 1/8 that of the original tube. b. Tube 4 is the 8x dilution tube. The concentration of methylene blue in this tube is 8 times that of the original tube. c. Tube 4 is the 4x dilution tube. The concentration of methylene blue in this tube is 4 times that of the original tube. d. Tube 4 is the dilution 10x tube. The concentration of methylene blue in this tube is 1/10 that of the original tube. e. None of the above
Penicillin G is a natural antibiotic that is useful for treating infections caused by Gram positive bacteria. What is the functional benefit of the semi-synthetic antimicrobials carbenicillin and ampicillin, generated by chemical modification of the R group so their R groups each are a bit different than the R group seen with penicillin G?
a)What Group? (Carb, Lipid, Protein, or Nucleic b)Acid). Within the group, how would you classify it? 1. Molecule #1 a) CH2OH H CH2OH b) H HỌ CH2OH он н он ÓH H c)How do you know you correctly classified this macromolecule in its proper group?
20. Since heating is used to deodorize oils in the refining process, what can you conclude about the size and saturation of the odorant molecules relative to fatty acids? A) Odorant molecules should be larger and/or less saturated than typical fatty acids B) Odorant molecules should be smaller and/or less saturated than typical fatty acids C) Odorant molecules should be smaller and/or more saturated than typical fatty acids D) Odorant molecules should be larger and/or more saturated than typical fatty acids
This lab examines the relationship between the absorbance of light by a solution at 595 nm and the concentration of the Coomassie Blue dye-BSA protein complex in the solution. State whether the following descriptions of the lab experiment are valid or not, and explain why you say Yes or No: a.The experiment would be significantly more accurate if absorbance readings were recorded for a range of wavelengths, not just for 595 nm.b.The experiment has limited accuracy because it does not account for the absorbance of light by the other components (components that are not the dye-protein complex, such as excess dye that is not bound to any protein) of the solution.c.The absorbance reading measures practically all the protein content in the solutions
4. Molecule #4 a)What Group? (Carb, Lipid, Protein, or Nucleic Acid). b)Within the group, how would you classify it? a) -NH b) "NH2 но-р-о онон c)How do you know you correctly classified this macromolecule in its proper group? O=a-o
provide detailed explanation.
6. Which class or reactions results in the degradation of all macromolecules: a) dehydration synthesis of monomersà polymers with the loss of an H2O with the addition of each monomer b) hydrolysis with the addition of one H2O for each reaction c) oxidation with the loss of an e- d) reduction with the gain of an e-
Why do proteins absorb light?

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9.) Riboflavin is an interesting B-vitamin (Vitamin B2) with the given structure. Could you use the following techniques to detect riboflavin? For each answer give a short sentence why or why not: CH3 NH CH 3 A.) Fluorescence HO B.) UV-Vis absorbance HO. C.) GC-FID D.) ESI (with quadrupole)