5. The following are short answer questions concerning chromatography. a. b. C. 2 In GC, you learned that H₂ gas has some advantages over using He gas. What are two advantages? If you are using uv/vis absorption to detect analytes coming off a chromatography column, why are you more likely to measure peak area that peak height when making a graph of signal versus concentrations for the standards? The van Deemter equation can be written as: H = A + B/u + Cu Give brief explanations for the A, B, and C terms. Be sure to explain how each term is affected by the flow rate of the mobile phase.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
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5.
The following are short answer questions concerning
chromatography.
a.
b.
C.
In GC, you learned that H, gas has some
advantages over using He gas. What are two advantages?
If you are using uv/vis absorption to detect
analytes coming off a chromatography column, why are
you more likely to measure peak area that peak height
when making a graph of signal versus concentrations for
the standards?
The van Deemter equation can be written as:
H = A + B/u + Cu
Give brief explanations for the A, B, and C terms. Be sure
to explain how each term is affected by the flow rate of the
mobile phase.
Transcribed Image Text:5. The following are short answer questions concerning chromatography. a. b. C. In GC, you learned that H, gas has some advantages over using He gas. What are two advantages? If you are using uv/vis absorption to detect analytes coming off a chromatography column, why are you more likely to measure peak area that peak height when making a graph of signal versus concentrations for the standards? The van Deemter equation can be written as: H = A + B/u + Cu Give brief explanations for the A, B, and C terms. Be sure to explain how each term is affected by the flow rate of the mobile phase.
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