5 Any Cu* ions present in solution will react with FerroZine in the same way as Fe2+. a. What effect would the presence of Cu²* in a patient's blood sample have on the determination of serum iron concentration? Explain. - Neocuproine is a compound that reacts selectively with Cu²+ and may be used to remove these ions from a patient sample, thereby preventing any interference with the determination of serum iron. Should the neocuproine be added to the patient sample before or after FerroZine? Explain your answer.

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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a. What effect would the presence of Cu2+ in a patient's blood sample have on the determination of serum iron
concentration? Explain.
5 Any Cu+ jons present in solution will react with FerroZine in the same way as Fe2+.
b. Neocuproine is a compound that reacts selectively with Cu2+ and may be used to remove these ions from a patient
sample, thereby preventing any interference with the determination of serum iron. Should the neocuproine be added
to the patient sample before or after FerroZine? Explain your answer.
6 The concentration of inorganic phosphate (PO,3-), another important ion in the body, may be determined by
spectrophotometric analysis. The phosphate is reacted with a compound to produce a deep-blue species that strongly
absorbs light at 650 nm. For an adult, normal phosphate levels are 2.4-4.4 mg/dL. Use the following data to prepare
a calibration curve, and then determine the concentration of phosphate for a patient whose sample gave an absorbance
value of 0.782. Is this patient's level within the normal range? Include a plot of your computer generated calibration
curve and show your work. Assume all samples were analyzed in a 1.0 cm pathlength cuvette.
PO,3- Concentration (mg/dL)
Absorbance at 562 nm
1.7
0.295
2.2
0.380
3.1
0.534
4.0
0.688
5.5
0.945
Transcribed Image Text:a. What effect would the presence of Cu2+ in a patient's blood sample have on the determination of serum iron concentration? Explain. 5 Any Cu+ jons present in solution will react with FerroZine in the same way as Fe2+. b. Neocuproine is a compound that reacts selectively with Cu2+ and may be used to remove these ions from a patient sample, thereby preventing any interference with the determination of serum iron. Should the neocuproine be added to the patient sample before or after FerroZine? Explain your answer. 6 The concentration of inorganic phosphate (PO,3-), another important ion in the body, may be determined by spectrophotometric analysis. The phosphate is reacted with a compound to produce a deep-blue species that strongly absorbs light at 650 nm. For an adult, normal phosphate levels are 2.4-4.4 mg/dL. Use the following data to prepare a calibration curve, and then determine the concentration of phosphate for a patient whose sample gave an absorbance value of 0.782. Is this patient's level within the normal range? Include a plot of your computer generated calibration curve and show your work. Assume all samples were analyzed in a 1.0 cm pathlength cuvette. PO,3- Concentration (mg/dL) Absorbance at 562 nm 1.7 0.295 2.2 0.380 3.1 0.534 4.0 0.688 5.5 0.945
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