43. The recovery of fluorescence in the photobleached area is due to the fact that_

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Questions 43-50
You are investigating the properties of cell
membranes using the Fluorescence Recovery After
Photobleaching (FRAP) technique. You first label all
the membrane proteins on the cell surface uniformly
with a green fluorescent dye. At 10 minutes, you
photobleach a small area on the cell membrane by
shining a beam of strong laser on it (Strong laser
permanently destroys the fluorescent molecules in
the area). You measure the fluorescence in the
photobleached area over time.
10min
Time
43. The recovery of fluorescence in the
photobleached area is due to the fact that .
A. The cell membrane is semi-permeable, allowing
proteins to be transported across the membrane.
B. The cell membrane is fluid, allowing proteins
from adjacent areas to move into the
photobleached area.
C. Fluorescent molecules are not permanently
destroyed by the laser beam. They can
spontaneously regain their fluorescence over time.
D. New proteins are synthesized in the cell and
transported to the cell surface.
Fluorescence in the
bleached area
Transcribed Image Text:Questions 43-50 You are investigating the properties of cell membranes using the Fluorescence Recovery After Photobleaching (FRAP) technique. You first label all the membrane proteins on the cell surface uniformly with a green fluorescent dye. At 10 minutes, you photobleach a small area on the cell membrane by shining a beam of strong laser on it (Strong laser permanently destroys the fluorescent molecules in the area). You measure the fluorescence in the photobleached area over time. 10min Time 43. The recovery of fluorescence in the photobleached area is due to the fact that . A. The cell membrane is semi-permeable, allowing proteins to be transported across the membrane. B. The cell membrane is fluid, allowing proteins from adjacent areas to move into the photobleached area. C. Fluorescent molecules are not permanently destroyed by the laser beam. They can spontaneously regain their fluorescence over time. D. New proteins are synthesized in the cell and transported to the cell surface. Fluorescence in the bleached area
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