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42. A mixture of A and B was partially separated on an HPLC The absorbance of the effluent was monitored at 310 nm column, resulting in overlap of their chromagraphic peaks. 1600) and 330 nm (EA = 2100, (EA 1800, EB E 3200); E is the molar absorptivity in L-moll.cm-¹. The absorbance at the maximum of the first chromatographic peak at 310 nm was 0.020 and at 330 nm was 0.030. What is the major component of the first eluting peak and what percentage of the total absorbance at 310 nm is due to this component? (A) A, 60% (C) A, 40% (B) B. 60% (D) B, 40%

42. A mixture of A and B was partially separated on an HPLC The absorbance of the effluent was monitored at 310 nm column, resulting in overlap of their chromagraphic peaks. 1600) and 330 nm (EA = 2100, (EA 1800, EB E 3200); E is the molar absorptivity in L-moll.cm-¹. The absorbance at the maximum of the first chromatographic peak at 310 nm was 0.020 and at 330 nm was 0.030. What is the major component of the first eluting peak and what percentage of the total absorbance at 310 nm is due to this component? (A) A, 60% (C) A, 40% (B) B. 60% (D) B, 40%

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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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42. A mixture of A and B was partially separated on an HPLC The absorbance of the effluent was monitored at 310 nm column, resulting in overlap of their chromagraphic peaks. (EA = 1800, E = 1600) and 330 nm (EA 2100, EB 3200); E is the molar absorptivity in L-molcm-¹. chromatographic peak at 310 nm was 0.020 and at 330 nm The absorbance at the maximum of the first was 0.030. What is the major component of the first eluting peak and what percentage of the total absorbance at 310 nm is due to this component? (A) A, 60% (C) A, 40% (B) B. 60% (D) B, 40% 43. The isothermal gas chromatographic separation of a mixture of three compounds produced retention times of 0.95 min, 1.15 min, and 8.40 min. The best means to shorten the analysis time without sacrifice of resolution would be to use (A) a longer chromatographic column. (B) a column operated at a higher isothermal temperature. (C) temperature programming of the column. (D) increased flow rate of the mobile phase in the column.
Transcribed Image Text:42. A mixture of A and B was partially separated on an HPLC The absorbance of the effluent was monitored at 310 nm column, resulting in overlap of their chromagraphic peaks. (EA = 1800, E = 1600) and 330 nm (EA 2100, EB 3200); E is the molar absorptivity in L-molcm-¹. chromatographic peak at 310 nm was 0.020 and at 330 nm The absorbance at the maximum of the first was 0.030. What is the major component of the first eluting peak and what percentage of the total absorbance at 310 nm is due to this component? (A) A, 60% (C) A, 40% (B) B. 60% (D) B, 40% 43. The isothermal gas chromatographic separation of a mixture of three compounds produced retention times of 0.95 min, 1.15 min, and 8.40 min. The best means to shorten the analysis time without sacrifice of resolution would be to use (A) a longer chromatographic column. (B) a column operated at a higher isothermal temperature. (C) temperature programming of the column. (D) increased flow rate of the mobile phase in the column.
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