4. To prepare a 1/25-dilution (25-fold dilution) of an enzyme solution at a final volume of 5 nor mls: volume of the enzyme solution is added to volume of diluent. 5. Show a two or three step serial dilution for a 1/250-dilution of an enzyme solution. 6. How many grams of glucose (180g/mole or 180 dalton) are needed to prepare a 250 ml solution with a final glucose concentration of 10 mM?
4. To prepare a 1/25-dilution (25-fold dilution) of an enzyme solution at a final volume of 5 nor mls: volume of the enzyme solution is added to volume of diluent. 5. Show a two or three step serial dilution for a 1/250-dilution of an enzyme solution. 6. How many grams of glucose (180g/mole or 180 dalton) are needed to prepare a 250 ml solution with a final glucose concentration of 10 mM?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:4. To prepare a 1/25-dilution (25-fold dilution) of an enzyme solution at a final volume of 5
yer mls:
120
volume of the enzyme solution is added to
volume of diluent.
5. Show a two or three step serial dilution for a 1/250-dilution of an enzyme solution.
6. How many grams of glucose (180g/mole or 180 dalton) are needed to prepare a 250 ml
solution with a final glucose concentration of 10 mM?
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