↓ 4. Predict what mode of action this antibiotic might use to kill the bacterial cell. You decide to test the new drug to see what mode of action the drug might use. You set up two sets of broths: one set you will allow to grow as a control; to the other broth, you add your new drug. You then take several measurements to attempt to determine the mode of action. Below are the measurements you make: ▪ Radioactive thymine incorporation (Figure 1) ▪ Radioactive methionine incorporation (Figure 2) ▪ Radioactive uracil incorporation (Figure 3) ▪ Glucose depletion (Figure 4) ▪ DNA present in the broth (Figure 5)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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4. Predict what mode of action this antibiotic might use to kill the bacterial cell. You decide to test the
new drug to see what mode of action the drug might use. You set up two sets of broths: one set you will
allow to grow as a control; to the other broth, you add your new drug. You then take several
measurements
to attempt to determine the mode of action. Below are the measurements you make:
▪ Radioactive thymine incorporation (Figure 1)
▪ Radioactive methionine incorporation (Figure 2)
▪ Radioactive uracil incorporation (Figure 3)
▪ Glucose depletion (Figure 4)
DNA present in the broth (Figure 5) ©
■
Transcribed Image Text:↓ 4. Predict what mode of action this antibiotic might use to kill the bacterial cell. You decide to test the new drug to see what mode of action the drug might use. You set up two sets of broths: one set you will allow to grow as a control; to the other broth, you add your new drug. You then take several measurements to attempt to determine the mode of action. Below are the measurements you make: ▪ Radioactive thymine incorporation (Figure 1) ▪ Radioactive methionine incorporation (Figure 2) ▪ Radioactive uracil incorporation (Figure 3) ▪ Glucose depletion (Figure 4) DNA present in the broth (Figure 5) © ■
Po
~Av
Αν
Radioactive Thymine
(գլոսյա/siunos)
500
400-
300-
200-
100-
Radioactive Uracil
(counts/minute)
Figure 1- Radioactive Thymine
E-621 3¶
= = = = 112
0-
100
80-
60-
40-
20
15
20
Time (minutes)
Figure 3-Radioactive Uracil
1
1
0-
14
ted States) IT
1
5
10
5
2
10
25
25
20
15
Time (minutes)
1
30
111111
30
V
g
DNA in broth
35
2-
1.5-
1-
0.5-
0
3
35
Test broth with antibiotic added
Control broth with no antibiotic added
1
V
40
glucose concentration to kill the bacteria.
40
Figure 5-DNA in the Broth
2.5-
5
L
AaBbCcDdEe AaBbCcDdEe AaBb AaBbC
Normal
No Spacing
Heading 1
Heading 2
Radioactive Methionine
10
(counts/minute)
30,000
25,000-
20,000-
15,000-
Figure 2- Radioactive Methionine
10,000-
5,000-
0++
500-
400-
300-
200-
100-
Figure 4- Glucose Concentration
0
25
1
15 20
Time (minutes)
LL
MacBook Pro
1
30
5
5
f
5
35
W
20 25
15
10
Time (minutes)
10
40
6
LI
25
15 20
Time (minutes)
30
30
35
35
40
40
Foc
Transcribed Image Text:Po ~Av Αν Radioactive Thymine (գլոսյա/siunos) 500 400- 300- 200- 100- Radioactive Uracil (counts/minute) Figure 1- Radioactive Thymine E-621 3¶ = = = = 112 0- 100 80- 60- 40- 20 15 20 Time (minutes) Figure 3-Radioactive Uracil 1 1 0- 14 ted States) IT 1 5 10 5 2 10 25 25 20 15 Time (minutes) 1 30 111111 30 V g DNA in broth 35 2- 1.5- 1- 0.5- 0 3 35 Test broth with antibiotic added Control broth with no antibiotic added 1 V 40 glucose concentration to kill the bacteria. 40 Figure 5-DNA in the Broth 2.5- 5 L AaBbCcDdEe AaBbCcDdEe AaBb AaBbC Normal No Spacing Heading 1 Heading 2 Radioactive Methionine 10 (counts/minute) 30,000 25,000- 20,000- 15,000- Figure 2- Radioactive Methionine 10,000- 5,000- 0++ 500- 400- 300- 200- 100- Figure 4- Glucose Concentration 0 25 1 15 20 Time (minutes) LL MacBook Pro 1 30 5 5 f 5 35 W 20 25 15 10 Time (minutes) 10 40 6 LI 25 15 20 Time (minutes) 30 30 35 35 40 40 Foc
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