3. Consider Anfinsen's classical protein folding experiment. SH G=A Native (100% active) Ğ Native 1. Reduce 2.8 M urea 1. Reduce 2.8 M urea SH SH HS Denatured (inactive) SH SH SH SH SH Denatured 1. Remove urea 2. Oxidize 1. Oxidize 2. Remove urea A B a. What do you expect to be the results of Experiment A versus Experiment B above. Explain. b. In Anfinsen's experiment, the Ribonuclease protein will fold correctly even if kept in a reducing environment (all disulfide bonds broken). What are the main driving forces that lead to proper folding? c. What is the main force that opposes folding?
3. Consider Anfinsen's classical protein folding experiment. SH G=A Native (100% active) Ğ Native 1. Reduce 2.8 M urea 1. Reduce 2.8 M urea SH SH HS Denatured (inactive) SH SH SH SH SH Denatured 1. Remove urea 2. Oxidize 1. Oxidize 2. Remove urea A B a. What do you expect to be the results of Experiment A versus Experiment B above. Explain. b. In Anfinsen's experiment, the Ribonuclease protein will fold correctly even if kept in a reducing environment (all disulfide bonds broken). What are the main driving forces that lead to proper folding? c. What is the main force that opposes folding?
Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Transcribed Image Text:3. Consider Anfinsen's classical protein folding experiment.
SH
G=A
Native
(100% active)
Ğ
Native
1. Reduce
2.8 M urea
1. Reduce
2.8 M urea
SH
SH
HS
Denatured
(inactive)
SH
SH
SH
SH
SH
Denatured
1. Remove urea
2. Oxidize
1. Oxidize
2. Remove urea
A
B
a. What do you expect to be the results of Experiment A versus Experiment B above. Explain.
b. In Anfinsen's experiment, the Ribonuclease protein will fold correctly even if kept in a reducing
environment (all disulfide bonds broken). What are the main driving forces that lead to proper
folding?
c. What is the main force that opposes folding?
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