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2. Using your notes on the citric acid cycle, What is the name of the enzyme that does the following? Add the necessary cofactors for this reaction and any molecules that add/leave. Succinate Succinyl-CoA o S-COA Draw the mechanism of the enzyme showing the different reactions. (This is probably more detail than necessary for an exam, but it gets you practicing redox arrows).

2. Using your notes on the citric acid cycle, What is the name of the enzyme that does the following? Add the necessary cofactors for this reaction and any molecules that add/leave. Succinate Succinyl-CoA o S-COA Draw the mechanism of the enzyme showing the different reactions. (This is probably more detail than necessary for an exam, but it gets you practicing redox arrows).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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### Enzyme Reaction in the Citric Acid Cycle #### Background: Hemiacetal Structure - A hemiacetal is depicted: - General structure: - R - \ - H—C—OH - / - OH #### Enzyme Reaction Task **Question 2:** Using your notes on the citric acid cycle, identify the enzyme facilitating the reaction below. Include necessary cofactors and any additional molecules involved. **Reaction Overview:** - **Left Side:** Succinate - **Reaction Type:** Conversion to Succinyl-CoA - **Right Side:** Succinyl-CoA - **Molecule Added:** Coenzyme A (CoA) - **Product Released:** Proton (H⁺) **Instruction:** Draw the enzyme-catalyzed mechanism illustrating the process. Practice using redox arrows to help understand the reaction steps. This exercise may include more detail than required for exams but facilitates learning. **Detailed Mechanism:** - **Initial State:** Succinate is ready to react. - **Reaction Intermediate:** - Succinate interacts with HS-CoA. - An arrow indicates the introduction of Coenzyme A. - **Final Product:** - Succinyl-CoA is formed. - A proton (H⁺) is released as a byproduct. #### Diagram Explanation 1. **Succinate:** Depicted with oxygen double bonds on two ends of the molecule. 2. **Reaction Arrow:** Indicates conversion to Succinyl-CoA. 3. **Succinyl-CoA:** Shown with an S-CoA group replacing one oxygen. This reaction is a critical part of the citric acid cycle, emphasizing the addition of CoA and the release of a proton, crucial for cellular metabolism and energy production.
Transcribed Image Text:### Enzyme Reaction in the Citric Acid Cycle #### Background: Hemiacetal Structure - A hemiacetal is depicted: - General structure: - R - \ - H—C—OH - / - OH #### Enzyme Reaction Task **Question 2:** Using your notes on the citric acid cycle, identify the enzyme facilitating the reaction below. Include necessary cofactors and any additional molecules involved. **Reaction Overview:** - **Left Side:** Succinate - **Reaction Type:** Conversion to Succinyl-CoA - **Right Side:** Succinyl-CoA - **Molecule Added:** Coenzyme A (CoA) - **Product Released:** Proton (H⁺) **Instruction:** Draw the enzyme-catalyzed mechanism illustrating the process. Practice using redox arrows to help understand the reaction steps. This exercise may include more detail than required for exams but facilitates learning. **Detailed Mechanism:** - **Initial State:** Succinate is ready to react. - **Reaction Intermediate:** - Succinate interacts with HS-CoA. - An arrow indicates the introduction of Coenzyme A. - **Final Product:** - Succinyl-CoA is formed. - A proton (H⁺) is released as a byproduct. #### Diagram Explanation 1. **Succinate:** Depicted with oxygen double bonds on two ends of the molecule. 2. **Reaction Arrow:** Indicates conversion to Succinyl-CoA. 3. **Succinyl-CoA:** Shown with an S-CoA group replacing one oxygen. This reaction is a critical part of the citric acid cycle, emphasizing the addition of CoA and the release of a proton, crucial for cellular metabolism and energy production.
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