2. Below, recognition sites of two of these enzymes, Pstl and Nsil, are given 5'... CTGCAG...3 3... GA CG TC...5 Pstl recognition and cleavage site 5'... ATGCAT ...3 3'... TACGT A... 5 Nsil recognition and cleavage site Does cleavage by Pstl result in a 5' or 3' overhang? What is the sequence of this overhang? b. Does cleavage by Nsil result in a 5' or 3' overhang? What is the sequence of this overhang? c. Suppose you have a cloning vector that contains a Pstl recognition site and you also have foreign DNA that was cut with Nsil. Can this DNA be ligated into the Pstl site of the vector, and if so, explain its reason and draw the new ligation site. d. Can the new ligated DNA segment sequence be cut from the vector with Pstl or Nsil ? What potential problems do you see?

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2. Below, recognition sites of two of these enzymes, Pstl and Nsil, are given
5'... CTGC AG.
3... GACGTC...5
3
Pstl recognition and cleavage site
5... ATGCAT
3'... TACGTA ... 5'
3'
...
Nsil recognition and cleavage site
a. Does cleavage by Pstl result in a 5' or 3' overhang? What is the sequence of this
overhang?
b. Does cleavage by Nsil result in a 5' or 3' overhang? What is the sequence of this
overhang?
c. Suppose you have a cloning vector that contains a Pstl recognition site and you also
have foreign DNA that was cut with Nsil. Can this DNA be ligated into the Pstl site of
the vector, and if so, explain its reason and draw the new ligation site.
d. Can the new ligated DNA segment sequence be cut from the vector with Pstl or Nsil ?
What potential problems do you see?
Transcribed Image Text:2. Below, recognition sites of two of these enzymes, Pstl and Nsil, are given 5'... CTGC AG. 3... GACGTC...5 3 Pstl recognition and cleavage site 5... ATGCAT 3'... TACGTA ... 5' 3' ... Nsil recognition and cleavage site a. Does cleavage by Pstl result in a 5' or 3' overhang? What is the sequence of this overhang? b. Does cleavage by Nsil result in a 5' or 3' overhang? What is the sequence of this overhang? c. Suppose you have a cloning vector that contains a Pstl recognition site and you also have foreign DNA that was cut with Nsil. Can this DNA be ligated into the Pstl site of the vector, and if so, explain its reason and draw the new ligation site. d. Can the new ligated DNA segment sequence be cut from the vector with Pstl or Nsil ? What potential problems do you see?
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