1C. You sporulate the diploid yeast created by mating Haploid 1 and Haploid 2 and examine the phenotypes of all the spores. Tetrads are numbered from 1-12 and spores within a tetrad are labeled A-D. All spores were able to grow on medium with both methionine and tryptophan added back (not shown). - MET - TRP AB C D AB C D 1 1 2 2 3 3

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Please solve 1C,1D,1E and 1F
1. These questions will utilize yeast analysis and provide you with mock experimental
data for the phenotypic analysis of yeast growth to infer genotypes. The following
symbols indicate growth or no growth on medium lacking particular components
(examples in 1A are medium lacking methionine "-MET" and medium lacking tryptophan
"-TRP". Growth is indicated by a filled, black circle. No growth on a particular medium is
indicated by an unfilled circle. In cases where we look viability of spores, we would use
absence of a circle to indicate that a spore did not grow at all (for example, it inherited a
lethal mutation).
Growth No growth
1A. You have two haploid strains (Haploid of opposite mating types. Their genotypes
and phenotypes are shown below.
+MET
TRP - MET +TRP
Haploid 1 MATA: MET2 trp3
Haploid 2 MATalpha: met2 TRP3
You cross each strain to a WT yeast and observe the following:
Haploid 1 x WT= Growth on all three media shown above.
Haploid 2 x WT= Growth on all three media shown above.
What do you conclude about met2 and trp3. (select all correct answers)
A. They are both dominant.
B. They are both recessive.
C. They are both complemented by WT.
D. WT is dominant to met2 and trp3.
E. met2 and trp3 are in the same gene.
Answer:
1B. You cross Haploid 1 to Haploid 2 to each other. The resulting diploid grows on all
three media shown above. What do you conclude? (select all correct answers)
A. They are both dominant.
B. They are both recessive.
C. The strains complement each other.
D. met2 and trp3 are in the same gene.
Answer:
1C. You sporulate the diploid yeast created by mating Haploid 1 and Haploid 2 and
examine the phenotypes of all the spores. Tetrads are numbered from 1-12 and spores
within a tetrad are labeled A-D. All spores were able to grow on medium with both
methionine and tryptophan added back (not shown).
- MET
- TRP
AB C D
АвсD
8
Transcribed Image Text:1. These questions will utilize yeast analysis and provide you with mock experimental data for the phenotypic analysis of yeast growth to infer genotypes. The following symbols indicate growth or no growth on medium lacking particular components (examples in 1A are medium lacking methionine "-MET" and medium lacking tryptophan "-TRP". Growth is indicated by a filled, black circle. No growth on a particular medium is indicated by an unfilled circle. In cases where we look viability of spores, we would use absence of a circle to indicate that a spore did not grow at all (for example, it inherited a lethal mutation). Growth No growth 1A. You have two haploid strains (Haploid of opposite mating types. Their genotypes and phenotypes are shown below. +MET TRP - MET +TRP Haploid 1 MATA: MET2 trp3 Haploid 2 MATalpha: met2 TRP3 You cross each strain to a WT yeast and observe the following: Haploid 1 x WT= Growth on all three media shown above. Haploid 2 x WT= Growth on all three media shown above. What do you conclude about met2 and trp3. (select all correct answers) A. They are both dominant. B. They are both recessive. C. They are both complemented by WT. D. WT is dominant to met2 and trp3. E. met2 and trp3 are in the same gene. Answer: 1B. You cross Haploid 1 to Haploid 2 to each other. The resulting diploid grows on all three media shown above. What do you conclude? (select all correct answers) A. They are both dominant. B. They are both recessive. C. The strains complement each other. D. met2 and trp3 are in the same gene. Answer: 1C. You sporulate the diploid yeast created by mating Haploid 1 and Haploid 2 and examine the phenotypes of all the spores. Tetrads are numbered from 1-12 and spores within a tetrad are labeled A-D. All spores were able to grow on medium with both methionine and tryptophan added back (not shown). - MET - TRP AB C D АвсD 8
- MET
- TRP
AB C D
AB C D
10
11
12
120
dpoo
What is the type of each tetrad?
A. PD. B. NPD. C. Tetratype. D. None of the above.
Question
Tetrad number
Tetrad type?
1C-1
1.
Answer:
1C-2
2
Answer:
1C-3
3
Answer:
1C-4
Answer:
1C-5
Answer:
1C-6
6
Answer:
1C-7
7
Answer:
1C-8
8
Answer:
1C-9
9
Answer:
1C-10
10
Answer:
1C-11
11
Answer:
1C-12
12
Answer:
1D. Are the two genes linked?)
A. Yes. B. No. C. Can't tell
Answer:
1E. Why or why not? (2 points)
1F. Is at least one of the genes centromere linked? (1 point)
A. Yes. B. No. C. Can't tell
||
Transcribed Image Text:- MET - TRP AB C D AB C D 10 11 12 120 dpoo What is the type of each tetrad? A. PD. B. NPD. C. Tetratype. D. None of the above. Question Tetrad number Tetrad type? 1C-1 1. Answer: 1C-2 2 Answer: 1C-3 3 Answer: 1C-4 Answer: 1C-5 Answer: 1C-6 6 Answer: 1C-7 7 Answer: 1C-8 8 Answer: 1C-9 9 Answer: 1C-10 10 Answer: 1C-11 11 Answer: 1C-12 12 Answer: 1D. Are the two genes linked?) A. Yes. B. No. C. Can't tell Answer: 1E. Why or why not? (2 points) 1F. Is at least one of the genes centromere linked? (1 point) A. Yes. B. No. C. Can't tell ||
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