18-16. A compound with a molecular mass of 292.16 was dissolved in a 5-mL volumetric flask. A 1.00-mL aliquot was withdrawn, placed in a 10-mL volumetric flask, and diluted to the mark. The absorbance at 340 nm was 0.427 in a 1.000-cm cuvet. The molar absorptivity for this compound at 340 nm is ɛ340 = 6 130 M-1 cm-!. (a) Calculate the concentration of compound in the cuvet. (b) What was the concentration of compound in the 5-mL flask? (c) How many milligrams of compound were used to make the 5-mL solution?

18-16. A compound with a molecular mass of 292.16 was dissolved in a 5-mL volumetric flask. A 1.00-mL aliquot was withdrawn, placed in a 10-mL volumetric flask, and diluted to the mark. The absorbance at 340 nm was 0.427 in a 1.000-cm cuvet. The molar absorptivity for this compound at 340 nm is ɛ340 = 6 130 M-1 cm-!. (a) Calculate the concentration of compound in the cuvet. (b) What was the concentration of compound in the 5-mL flask? (c) How many milligrams of compound were used to make the 5-mL solution?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Suppose that a solution has an absorbance of 0.250 at a wavelength of 450 nm. If the concentration of the solution is 22 μM, what is the value of the molar absorptivity? The data were taken with a standard 1-cm cuvette. molar absorptivity in (L cm^−1 mol^-1):
SPECTROPHOTOMETRY Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R? value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be…
What is the IR and HNMRAndCNMR and mass
What is the molar absorptivity of a 2.50 x 10-4 M solution if its absorbance at 550 nm is 0.500? The sample is placed in the standard 1-cm quartz cuvette. 2.00 x 10-3 M-1cm-1 5.00 x 10-4 M-1cm-1 2.00 x 103 M-1cm-1 1.25 x 10-4 M-1cm-1
A sample in a1-cm cuvette gives an absorbance reading of 0.558. If the absorptivity for this sample is 15,000 L mol-1 cm-1, what is the molar concentration?
Polarities of analyte functional group increase in the order of hydrocarbon < ethers < esters < ketones < aldehydes < amides < amines < alcohols Arrange the order of elution [from shortest to longest retention time] for the following six compounds from an HPLC C18 column. C2H5OH CH3COCH3 CH3COOH CH3COOC2H5 C2H50C2H5 (СН3)2NH O A. C2H5OH < CH3COOH < (CH3)2NH < CH3COCH3 < CH3COOC2H5
Maria was tasked to determine the concentration of a Fe2(SO4)3 solution. She prepared 5 different standards of Fe2(SO4)3 using the table below as her guide. She ran the standards and the unknown solution through UV-VIS spectroscopy and recorded the absorbances of each solution. HINT: use the dilution equation to determine the concentration of Fe2(SO4)3 in each test tube before preparing the standard curve. NOTE: No need to force the line to pass through zero. Just graph the data as is. What is the slope of the line of the standard curve from the given data? What is the concentration of the unknown solution?
You prepared a stock solution by dissolving a compound with the molar mass of 110.65 in a 100 mL volumetric flask. Then you removed a 2 mL aliquot and placed it in a 250 mL volumetric flask and dilute it to the mark. The absorbance of this diluted solution in the 250 mL volumetric flask at 288 nvm was 0.421 using a 2.50 cm path length curvet. The molar absorptivity for this compound at 288 nvm is 65000 M-1cm-1 How many ng of the compound were used to make the stock solution? a)1.44 b)0.96 c)0.72 d)1.22 e)2.87
A drug X (M.W. =300) has a molar absorptivity of 905 at 254 nm. One tablet containing this drug X is dissolved in water and diluted to a volume of 2 L. If the solution exhibits an absorbance of 0.655 in a 1-cm cell in the UV region at 254 nm, how many grams of X are contained in the tablet?
An unknown chemical has an absorbance of 0.884 in a 1.0cm cuvette ata concentration of 1.668 M. What is the chemical's absorptivity constant in M-cm? Report your answer to three decimals.
[Modified] A compound with molecular mass 427.35 g/mol was dissolved in a 5-mL volumetric flask. A 1.00-mL aliquot was withdrawn, placed in a 10-mL volumetric flask, and diluted to the mark. The absorbance at 340 nm was 0.431 in a 1.000-cm cuvet. The molar absorptivity at 340 nm is £340 = 6220 M-1.cm-1. (a) Calculate the concentration of compound in the cuvet. 4.0 6.93e-5 M (b) What was the concentration of compound in the 5-mL flask? 4.0 6.93e-4 M (c) How many milligrams of compound were used to make the 5-mL solution? 4.0 1.47 mg
What is the IR and HNMRAndCNMR and mass