12. As you recall, pUC18 is 2.7 kb in length and has a multiple cloning site (MCS) with a restriction order of EcoRI-BamHI-HindIII (EBH). Because my interest in the platypus is unending, a 12.3 kb fragment of platypus chromosome X5 was recombined into pUC18. The sequenced map is below. H E 2.6kb B EBH 2.7 kb 0.8 kb 2.8 kb 3.2 kb 2.9 kb B H Draw a labeled electrophoresis gel showing a ladder (500 bp, 1500 bp, 5000 bp present). undigested plasmid, single digests and double digests.

12. As you recall, pUC18 is 2.7 kb in length and has a multiple cloning site (MCS) with a restriction order of EcoRI-BamHI-HindIII (EBH). Because my interest in the platypus is unending, a 12.3 kb fragment of platypus chromosome X5 was recombined into pUC18. The sequenced map is below. H E 2.6kb B EBH 2.7 kb 0.8 kb 2.8 kb 3.2 kb 2.9 kb B H Draw a labeled electrophoresis gel showing a ladder (500 bp, 1500 bp, 5000 bp present). undigested plasmid, single digests and double digests.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 9P: Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram...

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Concept explainers

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.

Question

Please atleast answer this question

12. As you recall, pUC18 is 2.7 kb in length and has a multiple cloning site (MCS) with a
restriction order of EcoRI-BamHI-HindIll (EBH). Because my interest in the platypus is
unending, a 12.3 kb fragment of platypus chromosome X5 was recombined into pUC18.
The sequenced map is below.
H
E
2.6kb
B
EBH
2.7 kb
0.8 kb
2.8 kb
3.2 kb
2.9
kb
B
H
Draw a labeled electrophoresis gel showing a ladder (500 bp, 1500 bp, 5000 bp present),
undigested plasmid, single digests and double digests.

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