12. As you recall, pUC18 is 2.7 kb in length and has a multiple cloning site (MCS) with a restriction order of EcoRI-BamHI-HindIII (EBH). Because my interest in the platypus is unending, a 12.3 kb fragment of platypus chromosome X5 was recombined into pUC18. The sequenced map is below. H E 2.6kb B EBH 2.7 kb 0.8 kb 2.8 kb 3.2 kb 2.9 kb B H Draw a labeled electrophoresis gel showing a ladder (500 bp, 1500 bp, 5000 bp present). undigested plasmid, single digests and double digests.

12. As you recall, pUC18 is 2.7 kb in length and has a multiple cloning site (MCS) with a restriction order of EcoRI-BamHI-HindIII (EBH). Because my interest in the platypus is unending, a 12.3 kb fragment of platypus chromosome X5 was recombined into pUC18. The sequenced map is below. H E 2.6kb B EBH 2.7 kb 0.8 kb 2.8 kb 3.2 kb 2.9 kb B H Draw a labeled electrophoresis gel showing a ladder (500 bp, 1500 bp, 5000 bp present). undigested plasmid, single digests and double digests.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 9P: Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram...

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Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram the recombinational event leading to the formation of a heteroduplex DNA region within a bacteriophage chromosome.
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