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- #3The quenching of tryptophan fluorescence by dissolved O2 gas was monitored by measuring emission lifetimes at 348 nm in aqueous solutions. Determine the quenching rate constant for th is process from the followingdata:The variation of the molar Gibbs free pn- ergy of a substance with temperature and pressure is given by () T5/2 G = -T In Determine S, H, V, U and F.