1.0ml of a 1/10

Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line…

A: An isolated colony represents a pure source of an organism from which a pure culture can be started.

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: Your broth culture contains 471,612,048 bacteria/mL. Design a dilution series that would  result in…

A: To ensure an accurate count of bacterial colonies on agar plates, it's crucial to design a dilution…

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus…

A: A culture medium, also called the growth medium, can be a liquid or solid medium that supports the…

Q: in 5 hours, your bacterial culture went from a concentration of ~105 CFU/mL to ~1.6 x 106 CFU/mL,…

A: n=1.6x106 CFU/ml N=(no of cells at the beginning )=105 CFU/ml n=Nx2x x=no. of generation…

Q: Use the information in the tables below to determine the most probable number of bacteria per 100 mL…

A: Given  Number of positive tube  0.1 ml = 4  0.01 ml = 1  0.001 ml = 0

Q: RESULTS Results of the plate counts for the samples you worked with. Number of Colonies for Each…

A: Cfu/ml= (no. Of colonies plated× dilution factor)/volume plated.…

Q: Milk is usually pasteurized by a. the high-temperature short-time method c. batch method b.…

A: Pasteurization is the process, in which bacteria are killed and the sterilization is done to improve…

Q: A sterile powder vial label indicates to add 3.4 mL of sterile water which will produce a final…

A: Reconstitution of sterile powder vials is a common practice in healthcare settings where medications…

Q: Which of the following medium is considered an enriched medium? a. LB b. T-soy  c. Nutrient  d.…

A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…

Q: An inoculating loop must be flamed to uniform orange-hot from base to tip   A. after culture is…

A: An inoculation loop is a tool used by microbiologists to transfer a small culture of microorganisms.…

Q: You pipette 1mL from a 30mL starting culture into a 99mL blank labeled A. You plate 1mL and 0.1mL of…

A: This question spins around the concept of serial dilution and colony-forming units (CFU)…

Q: RODAC plates will be used for which exercise? Select ALL that apply. A. Soil microbe enumeration B.…

A: Introduction : Lecithin and polysorbate, which are components of RODAC plates, a modified TSA…

Q: You plated 0.1 ml of a sample of kombucha diluted by a factor of 10^-3. It produced 43 colonies.…

A: The serial dilution process is used to calculate the number of microorganisms (bacteria) within a…

Q: Which of the following medium would be most appropriate for the bacterial cultures from stomach? a.…

A: Microorganism culture out of their natural environment in pure form in the laboratory has been…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: Numeration After spread and pour plating, incubate plates inverted at 35 °C for 24-48 h, and…

A: To calculate the CFU/ml, we divide number of colonies by the final dilution. We are given two…

Q: Which isolation technique is best used in isolating microorganisms (in countable good plates) from a…

A: The phrase "isolation" in microbiology refers to the separation of a strain from a natural, mixed…

Q: What type of medium is best used to maintain a preserve specimen before clinical analysis? A.…

A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…

Q: Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml…

A: Serial dilution is a stepwise dilution of a substance in solution. Ordinarily, the dilution factor…

Q: A student plates 100 µL of a 107 dilution of E. coli culture on a Luria Agar plate. After 24 hour,…

A: The bacterial sample when taken from the stock solution, it may contain lots of bacterial cells, and…