1. Make the following solutions: Lysis buffer (100 mL): 0.1 M NaH2PO4, 10 mM Tris, 6 M Urea, pH 8.0 Dissolve the bulk reagents, adjust the pH with HCI as necessary, dilute to 500 mL Bradford Dye (500mL): 0.01% (w/v) Coomassie Blue G-250, 5% (v/v) ethanol, 8.5% phosphoric acid Dissolve Coomassie in ethanol, then add the phosphoric acid. Dilute to 500 mL and filter through #1 Watman filter paper. Bovine Serum Albumin: 1 mg/mL in diH20
1. Make the following solutions: Lysis buffer (100 mL): 0.1 M NaH2PO4, 10 mM Tris, 6 M Urea, pH 8.0 Dissolve the bulk reagents, adjust the pH with HCI as necessary, dilute to 500 mL Bradford Dye (500mL): 0.01% (w/v) Coomassie Blue G-250, 5% (v/v) ethanol, 8.5% phosphoric acid Dissolve Coomassie in ethanol, then add the phosphoric acid. Dilute to 500 mL and filter through #1 Watman filter paper. Bovine Serum Albumin: 1 mg/mL in diH20
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
![1. Make the following solutions:
Lysis buffer (100 mL): 0.1 M NaH,PO4, 10 mM Tris, 6 M Urea, pH 8.0
Dissolve the bulk reagents, adjust the pH with HCl as necessary, dilute to 500 ml
Bradford Dye (500mL): 0.01% (w/v) Coomassie Blue G-250, 5% (v/v) ethanol, 8.5% phosphoric acid
Dissolve Coomassie in ethanol, then add the phosphoric acid. Dilute to 500 mL
and filter through #1 Watman filter paper.
Bovine Serum Albumin: 1 mg/mL in diH20](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5bbd0284-8cc0-45b5-b1aa-cc674bd15e76%2F7855b1bf-7fd2-4e5b-bc0e-321e2cb7f4e8%2Fgdb0mu_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1. Make the following solutions:
Lysis buffer (100 mL): 0.1 M NaH,PO4, 10 mM Tris, 6 M Urea, pH 8.0
Dissolve the bulk reagents, adjust the pH with HCl as necessary, dilute to 500 ml
Bradford Dye (500mL): 0.01% (w/v) Coomassie Blue G-250, 5% (v/v) ethanol, 8.5% phosphoric acid
Dissolve Coomassie in ethanol, then add the phosphoric acid. Dilute to 500 mL
and filter through #1 Watman filter paper.
Bovine Serum Albumin: 1 mg/mL in diH20
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