solution (in water) and 0.2M Tris-HCl, pH=8.00. For this part, the concentration of PNPP will remain constant at 0.150mM. Fill the table below (before coming to lab) and use it to prepare your cuvettes. The completed table must be in your pre-lab. Your blank for each reaction is the PNPP mixed with Tris-HCL and Pi. Again, prepare one cuvette at the time to minimize exposure of the PNPP to the air. You can prepare your
Ionic Equilibrium
Chemical equilibrium and ionic equilibrium are two major concepts in chemistry. Ionic equilibrium deals with the equilibrium involved in an ionization process while chemical equilibrium deals with the equilibrium during a chemical change. Ionic equilibrium is established between the ions and unionized species in a system. Understanding the concept of ionic equilibrium is very important to answer the questions related to certain chemical reactions in chemistry.
Arrhenius Acid
Arrhenius acid act as a good electrolyte as it dissociates to its respective ions in the aqueous solutions. Keeping it similar to the general acid properties, Arrhenius acid also neutralizes bases and turns litmus paper into red.
Bronsted Lowry Base In Inorganic Chemistry
Bronsted-Lowry base in inorganic chemistry is any chemical substance that can accept a proton from the other chemical substance it is reacting with.
- Prepare another 5 cuvettes with different concentration of Pi using the stock 1mM Pi, 0.5mM PNPP solution (in water) and 0.2M Tris-HCl, pH=8.00. For this part, the concentration of PNPP will remain constant at 0.150mM. Fill the table below (before coming to lab) and use it to prepare your cuvettes. The completed table must be in your pre-lab. Your blank for each reaction is the PNPP mixed with Tris-HCL and Pi. Again, prepare one cuvette at the time to minimize exposure of the PNPP to the air. You can prepare your next cuvette while your previous one is being read.
- previous one is being read.
|
Final [Pi]uM |
Volume of 1mM Pi |
Final [PNPP] mM |
Volume of 0.5mM PNPP |
Volume of 0.2M Tris-HCl (uL) |
Volume of enzyme (uL) |
Total Volume (uL) |
|
0 |
0.150 |
100 |
1500 |
|||
|
3.33 |
0.150 |
100 |
1500 |
|||
|
20 |
0.150 |
100 |
1500 |
|||
|
50 |
0.150 |
100 |
1500 |
|||
|
100 |
0.150 |
100 |
1500 |
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