1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock? 2. How do you prepare 50 mL of a 0.25X solution of Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?
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1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock?
2. How do you prepare 50 mL of a 0.25X solution of
Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?
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- Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?1) You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed. 2. How would you make up one liter of 1X TAE buffer using a 25X stock?can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1LProportions§ 0.25M tris§ 1.92M glycine§ pH 8.3§ 1% (w/v) SDSInstructions to prepare 100mL of 10X running buffer1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir todissolve.2. Check the pH with a calibrated pH metre once dissolved.§ The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5.3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution.4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.
- How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? PLEASE GIVE THE MEASUREMENTSHow much of a 10x buffer should be added to 900ml of water to make a 1x solution?1. Prepare 30 mL of a 5 mg/mL oral phenobarbital solution using phenobarbital available from the manufacturer in a 65 mg/mL solution that comes in 1-mL vials. How much stock solution will be required? 2. What mass in milligrams of potassium nitrate is present in 0.25 kg of a 500 ppm aqueous solution of KNO,?
- 1) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula. 2)Calculate the required gram of EDTA to prepare O.5M, 100 ml EDTA (EDTA stock) mol and Molarity formula. 3.) Calculate the required volume of Tris Stock to prepare buffer A which contains 25 mM Tris-Hcl with final volume is 100 ml by using M1V1 =M2V2 formula 4) Calculate the required volume of EDTA stock to prepare buffer A which contains 10 mM EDTA with final volume is 100 ml by using M1V1 =M2V2 formula 5)Do the same for Buffer B calculationsHi, I just need some help calculating the final concentration for each of the standard solutions I made. The concentration of my stock solution is 0.1mM.To make 200 ml 0.4XSSC (Saline and Sodium Citrate) solution for FISH post-washing, how many ml 20X SSC solution is needed?
- To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?how to prepare a 100 mL of 4x sds buffer from a 1.0 L of 50x SDS stock solution. Show complete solution2. 3. Perform all the calculations necessary for the volume of stock solution or water, in mL, needed to make the dilute solutions listed below. a. b. C. Make: 200 mL of 30% Ethanol dilute solution Stock Volume needed (mL) 95% Ethanol Reagent water Make: 100mL of .09%NaCL Stock 1% NaCl Reagent water 30% SDS 3 M NaOH Make: 100 mL of a 3% SDS and 0.5 MNaOH dilute solution as follows: Stock Volume needed (mL) Reagent water 200 mL Total 1% Lactose 1% Dextrose Volume needed (mL) 100 mL Total Calculate the grams of chemical needed for each stock listed below. Concentration of Stock to Prepare Volume of Stock Needed 1% Sucrose 100 mL Total 10 mL 20 mL 25 mL Amount of chemical Needed (in grams) Your instructor will have you make ONE of the above solutions-ask your instructor which solution to make.