1. For enzyme catalyzed reaction: ki S + E =X (fast) k2 X -2> P + E (slow) derive the rate law and answer the following: a) What is meant by Michaelis-Menten constant km? (b) Prove that order of reaction in substrate changes from unity to zero at higher [S]. (c) What is the rate and value of km, if k2 >> k1. (d) Draw the potential energy diagram for enzyme catalyzed reaction.
1. For enzyme catalyzed reaction: ki S + E =X (fast) k2 X -2> P + E (slow) derive the rate law and answer the following: a) What is meant by Michaelis-Menten constant km? (b) Prove that order of reaction in substrate changes from unity to zero at higher [S]. (c) What is the rate and value of km, if k2 >> k1. (d) Draw the potential energy diagram for enzyme catalyzed reaction.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![1. For enzyme catalyzed reaction:
ki
S + E=X (fast)
k2
X -2> P + E (slow)
derive the rate law and answer the following:
a) What is meant by Michaelis-Menten constant km?
(b) Prove that order of reaction in substrate changes from unity to zero at
higher [S].
(c) What is the rate and value of km, if k2 >> k1.
(d) Draw the potential energy diagram for enzyme catalyzed reaction.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F957863ff-5279-4eda-a2a0-4d485ca39fc3%2Fe9ec71bc-e9b1-48ac-86fc-b2c98ada95e9%2Fs1k7q4i_processed.png&w=3840&q=75)
Transcribed Image Text:1. For enzyme catalyzed reaction:
ki
S + E=X (fast)
k2
X -2> P + E (slow)
derive the rate law and answer the following:
a) What is meant by Michaelis-Menten constant km?
(b) Prove that order of reaction in substrate changes from unity to zero at
higher [S].
(c) What is the rate and value of km, if k2 >> k1.
(d) Draw the potential energy diagram for enzyme catalyzed reaction.
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