Indicate for each organism if it is susceptible, Intermediate or Resistant. Answer the question 2 and 3. LABORATORY REPORT FORMEXERCISE 27ANTIBIOTIC SENSITIVITY TESTING: THE KIRBY-BAUER PROCEDUREWhat is the purpose of this exercise?To determine the sensitivity or resistance of pathogenic aerobic andcompounds.Facultative anaerobic bacteria to various antimicrobial1. Complete the following chart:CHEMOTHERAPEUTICAGENTDISKCODEStreptomycin 10NeomycinDoxydycline30Chloramphenicol 3010102.AmpicillinGentamicinBacitracin10Tetracycline 30Staphylococcus aureusS, I, or RZone(mm)1530 253D3242201530Escherichia coliZone(mm)28202819300S, I, or RPseudomonasaeruginosaZone(mm)10000020010S, I, or RBased on your results, which chemotherapeutic agent(s) tested were broad-spectrum? Narrow-spectrum?3. Were any colonies growing within any of your zones of inhibition? If so, how would you determine if itwas a contaminant or a resistant colony? antibiotics are onlyTable 27.1ANTIMICROBIALAGENTAmpicillinEnterobacteriaceaeStaphylococciEnterococciStreptococciBacitracinCephalothinChloramphenicolClindamycinErythromycinGentamicinKanamycinMethicillinNeomycinNitrojurantoinPenicillin GStaphylococciEnterococciStreptococciPolymyxin BRifampinInterpretive Standards for Disk-Diffusion Susceptibility TestingZONE OF INHIBITION (mm)DISK CONTENTIntermediateStreptomycinSulfisoxazole(Gantrisin)SulfonamidesTetracyclineVancomycinEnterococciOther gram (+)• Doxydycline10 μg10 units30 μg30 μg2 μg15 ug10 μg30 µg5 μg30 μg300 μg10 units300 units5 µg10 μg250 μg300 μg30 μg30 μgResistant≤13≤28≤16<21<8≤14< 12<14≤13≤12<13VI VI≤9< 12<14≤28≤14<19<8<16≤12≤12<12<14H9≤14<91214-1622-299-1215-1713-1715-2014-2213-1414-17,10-1313-1615-1620-279-1117-1912-1412-1613-1615-1815-1610-1113-15Susceptible217≥29≥17> 30≥13> 18218≥21≥23> 15218≥14≥17≥17229≥ 15≥28> 12>20> 15≥17≥17> 19≥17> 12216

The disk zone inhibition test is the sensitivity test for various bacterial species against specific…

1. Complete the following chart: CHEMOTHERAPEUTIC AGENT DISK CODE Streptomycin 10 Neomycin Doxydycline 30 Chloramphenicol 30 10 10 Staphylococcus aureus S, I, or R Ampicillin Gentamicin Bacitracin 10 Tetracycline 30 Zone (mm) 15 30 25 30 32 42 15 30 Escherichia coli Zone (mm) 0 28 20 28 19 30 0 24 S, I, or R Pseudomonas aeruginosa Zone (mm) 10 0 0 0 20 0 10 S, I, or R 2. Based on your results, which chemotherapeutic agent(s) tested were broad-spectrum? Narrow-spectrum? 3. Were any colonies growing within any of your zones of inhibition? If so, how would you determine if it was a contaminant or a resistant colony?

1. Complete the following chart: CHEMOTHERAPEUTIC AGENT DISK CODE Streptomycin 10 Neomycin Doxydycline 30 Chloramphenicol 30 10 10 Staphylococcus aureus S, I, or R Ampicillin Gentamicin Bacitracin 10 Tetracycline 30 Zone (mm) 15 30 25 30 32 42 15 30 Escherichia coli Zone (mm) 0 28 20 28 19 30 0 24 S, I, or R Pseudomonas aeruginosa Zone (mm) 10 0 0 0 20 0 10 S, I, or R 2. Based on your results, which chemotherapeutic agent(s) tested were broad-spectrum? Narrow-spectrum? 3. Were any colonies growing within any of your zones of inhibition? If so, how would you determine if it was a contaminant or a resistant colony?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

Suggest an approach you would take to identify the pharmacophore in the following leadcompound which inhibits poly(ADP-ribose) polymerase-1 (PARP-1).
What is a vector? What is a zoonotic pathogen? Example? (ha!)
Complete the colony morphology for the colonies pictured above: 1. Form - 2. Elevation - 3. Margin - 4. Surface - 5. Chromogenesis -
is selecting a colony from a blood agar plate tested with enterotube bad or good?
Citing the data from your results table tell which antibiotic is the most effective against this type of bacterium. Citing the data from the results table tell which antibiotic is the least effective against this type of bacterium. Explain how you determined the level of effectiveness. Describe what the Petri dish looked like. Hint(the most effective antibiotic has a larger clear area around the antibiotic disk. The least effective antibiotic has a smaller clear area around the antibiotic disk.
What method of bacterial measurement does this graph represent? Is it considered direct, or indirect? What phase of the growth curve can NOT be measured using this method?
Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample=number of bacteria/ml (For example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Based on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.
Look at these two plates. Which organism is most susceptible to antibiotics?
Based on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.
Bacteria have a single circular chromosome while human cells have 46 linear chromosomes. This means bacteria are considered half lloyd having only one copy of each gene.
Consider access of a digital extbook on the same computer being used for the exam as "leaving". If you are sing textbook notes/materials during a exam, they must be on non-electronic or n a separate device. ou will only have 1 attempt at this exam. D Question 20 Which of the following best represents a chemical mediator that inhibits pathogen invasion? O keratin cilia O sebum O serum O antibacterial soap O resident microbiota F O 2.5 pts hp Rain...