- Your current LTP experimental protocol is as follows:
- Isolate a ‘silent synapse’ with only NMDA receptors in the postsynaptic membrane.
- Induce LTP via low frequency presynaptic stimulation coupled with postsynaptic depolarization.
You’re able to verify that the synapse has been potentiated, as there are now strong post-synaptic responses to presynaptic stimulation. But you’re unable to prove conclusively that this change was AMPA receptor-mediated.
Explain how you will design an experiment to monitor the trafficking of AMPA receptors into the membrane during LTP using the electrophysiological tag, and how this technique works.
Explain how your method utilizes 1) Visualization and 2) Electrophysiological recordings to quantify trafficking of AMPA receptors.
Provide an example of how electrophysiological data can be interpreted support the hypothesis that LTP induction leads to the trafficking of AMPA receptors into the postsynaptic site.
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