1-proteins-lab-manual?module_item_id=44333 A= abc Where "A" is absorbance, "a" is molar absorptivity, "b" is the path length (length of the cuvette in units of cm), and "c" is concentration (mol/L). The molar absorptivity is an intrinsic property of a molecule which tells us how intensely it will absorb at a particular wavelength. If "A" is a unitless quantity, "b" is in units of cm, and "c" is in units of mol/L, then what are the units of molar absorptivity?

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macollege.instructure.com/courses/352/pages/covid-proteins-lab-manual?module_item_id=D44333
A = abc
Where "A" is absorbance, "a" is molar absorptivity, "b" is the path length (length of the cuvette in units of cm), and "c" is concentration
(mol/L). The molar absorptivity is an intrinsic property of a molecule which tells us how intensely it will absorb at a particular wavelength. If
"A" is a unitless quantity, "b" is in units of cm, and "c" is in units of mol/L, then what are the units of molar absorptivity?
Do a little research to learn how alpha-helices and beta-sheets are drawn in protein structures. If most of the absorption properties are
coming from the alpha-helices in these proteins, and many alpha-helices have similar absorbance properties across different proteins, which
protein would you predict would have the highest molar absorptivity?
Your job is to use the following data table to determine the molar absorptivity for each of the following proteins at their respective
wavelength of maximum absorbance, and record this information in your lab notebook (which you will submit online). Remember that molar
absorptivity is an intrinsic property of each protein, so you will get a single molar absorptivity for each of the three proteins. A 1 cm quartz
cuvette was used for each protein, and the absorbance values below are reported for the wavelength of maximum absorbance for each
protein. You should use Excel to assist you with this work, and you should include all three of your Excel plots in the lab notebook portion of
this lab that you will be submitting online. HINT: Remember that we should add a point at (0,0) since we always remember to calibrate our
spectrometer with a blank!
Protein
Abs. at c=0.11 M
Abs. at c=0.36 M
Abs. at c=0.76 M
0.06
0.15
0.33
Glycoprotein
0.44
0.93
1.45
Protease
1.15
2.14
0.53
RNA binding protein
Now that you have these molar absorptivity values, let's look at some samples from people who would like to get tested using the method we
have developed. We need to determine the concentration of SARS-Co\V-2 proteins given the following information for several patients that
have tested positive in other types of COVID-19 tests.
Patient 1: Upon examining for protease protein, it was found that the sample has an absorbance of 1.22 absorbance units in a 2 cm cuvette.
Patient 2: Upon examining for glycoprotein, it was found that the sample has an absorbance of 0.072 absorbance units in a 0.1 cm cuvette.
Patient 3: Upon examining for RNA binding protein, it was found that the sample has an absorbance of 1.45 absorbance units in a 0.5 cm
Please summarize your results for these patients in your summary report. Also address what you might see as potential benefits or problems
n natient hlond, Do you think we could examine a blood sample directly using this
cuvette.
Transcribed Image Text:macollege.instructure.com/courses/352/pages/covid-proteins-lab-manual?module_item_id=D44333 A = abc Where "A" is absorbance, "a" is molar absorptivity, "b" is the path length (length of the cuvette in units of cm), and "c" is concentration (mol/L). The molar absorptivity is an intrinsic property of a molecule which tells us how intensely it will absorb at a particular wavelength. If "A" is a unitless quantity, "b" is in units of cm, and "c" is in units of mol/L, then what are the units of molar absorptivity? Do a little research to learn how alpha-helices and beta-sheets are drawn in protein structures. If most of the absorption properties are coming from the alpha-helices in these proteins, and many alpha-helices have similar absorbance properties across different proteins, which protein would you predict would have the highest molar absorptivity? Your job is to use the following data table to determine the molar absorptivity for each of the following proteins at their respective wavelength of maximum absorbance, and record this information in your lab notebook (which you will submit online). Remember that molar absorptivity is an intrinsic property of each protein, so you will get a single molar absorptivity for each of the three proteins. A 1 cm quartz cuvette was used for each protein, and the absorbance values below are reported for the wavelength of maximum absorbance for each protein. You should use Excel to assist you with this work, and you should include all three of your Excel plots in the lab notebook portion of this lab that you will be submitting online. HINT: Remember that we should add a point at (0,0) since we always remember to calibrate our spectrometer with a blank! Protein Abs. at c=0.11 M Abs. at c=0.36 M Abs. at c=0.76 M 0.06 0.15 0.33 Glycoprotein 0.44 0.93 1.45 Protease 1.15 2.14 0.53 RNA binding protein Now that you have these molar absorptivity values, let's look at some samples from people who would like to get tested using the method we have developed. We need to determine the concentration of SARS-Co\V-2 proteins given the following information for several patients that have tested positive in other types of COVID-19 tests. Patient 1: Upon examining for protease protein, it was found that the sample has an absorbance of 1.22 absorbance units in a 2 cm cuvette. Patient 2: Upon examining for glycoprotein, it was found that the sample has an absorbance of 0.072 absorbance units in a 0.1 cm cuvette. Patient 3: Upon examining for RNA binding protein, it was found that the sample has an absorbance of 1.45 absorbance units in a 0.5 cm Please summarize your results for these patients in your summary report. Also address what you might see as potential benefits or problems n natient hlond, Do you think we could examine a blood sample directly using this cuvette.
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