1 2 3456 1-Control with no virus nucleic acid 2-Virus nucleic acid no treatment 3-Virus nucleic acid digested with Enzyme A 4-Virus nucleic acid digested with Enzyme B 5-Virus nucleic acid digested with Enzyme C 6-Virus nucleic acid base hydrolysis

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have isolated a new virus that you want to classify using the Baltimore approach to classification.  However, you don’t have much to work with.  Just an agarose gel, ethidium bromide to strain the nucleic acid on the gel, and a set of enzymes.   Assume you have pure virus.  Enzyme A digests ssDNA, Enzyme B digests all DNA, and Enzyme C digests ssRNA.  Base hydrolysis digests all RNA.  You run a gel and get the results below after staining with ethidium bromide:

From these results, what type of genome do you think the virus has?  Briefly explain your answer.

1 2 3456
1-Control with no virus nucleic acid
2-Virus nucleic acid no treatment
3-Virus nucleic acid digested with Enzyme A
4-Virus nucleic acid digested with Enzyme B
5-Virus nucleic acid digested with Enzyme C
6-Virus nucleic acid base hydrolysis
Transcribed Image Text:1 2 3456 1-Control with no virus nucleic acid 2-Virus nucleic acid no treatment 3-Virus nucleic acid digested with Enzyme A 4-Virus nucleic acid digested with Enzyme B 5-Virus nucleic acid digested with Enzyme C 6-Virus nucleic acid base hydrolysis
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