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An Illustrated Guide To Vet Med Term
4th Edition
ISBN:9781305465763
Author:ROMICH
Publisher:ROMICH
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Section: Chapter Questions
Problem 1FITB
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Experiment: Action of Gastric Proteases (contains pepsin and renin on proteins)

Procedure:

1. Prepare 3 test tubes and place 2 mL boiled egg albumin solution on each tube.

Tube 1: Add 1 mL Pepsin Solution
Tube 2: Add 1 mL of Pepsin + 2 drops conc. HCl
Tube 3: add nothing 

2. Keep all the test tubes in a beaker with hot water maintained at 40°C, for one hour or longer.

3. Filter the content of each test tube and perform Biuret’s Test by adding 2 mL 10% NaOH and 2 mL 
dilute CuSO4 solution to each test tube.

Question: What are the possible observations on each test tube? and What is its inference?

A.
Action of Gastric Proteases (contains pepsin and renin on proteins)
Test Tube
1
3
Observation
Inference
Transcribed Image Text:A. Action of Gastric Proteases (contains pepsin and renin on proteins) Test Tube 1 3 Observation Inference
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