Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below: • Fix in 1% OsO4 w/v in seawater for 1h • Transfer to distilled water, 6 changes over 12h • Store in 70% (v/v) ethanol There are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals. a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π h b) How much OsO4 do you need to weigh out to make your fixative? (in g)
Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below: • Fix in 1% OsO4 w/v in seawater for 1h • Transfer to distilled water, 6 changes over 12h • Store in 70% (v/v) ethanol There are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals. a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π h b) How much OsO4 do you need to weigh out to make your fixative? (in g)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below:
• Fix in 1% OsO4 w/v in seawater for 1h
• Transfer to distilled water, 6 changes over 12h
• Store in 70% (v/v) ethanol
There are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals.
a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π h
b) How much OsO4 do you need to weigh out to make your fixative? (in g)
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