. What is Chromatography? What kind of Chromatography did you use to separate food colors in this experiment? Chromatography is a widely used experimental lechnique by which a mixture of subslance con be seporoted into its single component. For this experiment we use Poper Chromatogrophy to separale food colors. 2. Define Rf value? Give its unit. KF is dehined as distonce hom origin to center of spot divided by distonce hom Ongin to solvent ront. Unit for Re valuc is Microwat 3. How did you identify the unknown sample?

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Can someone check my question and answers for chromatography lab I m also included data sheet of that lab
**QUESTIONS**

1. **What is Chromatography? What kind of Chromatography did you use to separate food colors in this experiment?**

   Chromatography is a widely used experimental technique by which a mixture of substances can be separated into its single components. For this experiment, we use Paper Chromatography to separate food colors.

2. **Define Rf value? Give its unit.**

   Rf is defined as the distance from the origin to the center of the spot divided by the distance from the origin to the solvent front.  
   Unit for Rf value is Microwatt.

3. **How did you identify the unknown sample?**

   *(No answer provided)*

4. **Is your felt-tip pen a mixture or a pure substance? How did you arrive at this conclusion?**

   No need to do this.

5. **Two food colors, A and B, have similar structure and similar polarities and a chromatogram for these compounds is illustrated here.**

   [Diagram explanation: The diagram shows a line titled "origin" on the left and "solvent front" on the right. Two spots, marked A and B, are placed along this line towards the origin, indicating two food colors.]

   **Which food color, A or B, would you predict to have the larger molecular weight?**

   Food Color A is having a larger molecular weight because food color that is close to the baseline is said to have more molecular weight.

6. **Give two real-life applications of chromatography.**

   1. Food Testing
   2. Drug Testing
Transcribed Image Text:**QUESTIONS** 1. **What is Chromatography? What kind of Chromatography did you use to separate food colors in this experiment?** Chromatography is a widely used experimental technique by which a mixture of substances can be separated into its single components. For this experiment, we use Paper Chromatography to separate food colors. 2. **Define Rf value? Give its unit.** Rf is defined as the distance from the origin to the center of the spot divided by the distance from the origin to the solvent front. Unit for Rf value is Microwatt. 3. **How did you identify the unknown sample?** *(No answer provided)* 4. **Is your felt-tip pen a mixture or a pure substance? How did you arrive at this conclusion?** No need to do this. 5. **Two food colors, A and B, have similar structure and similar polarities and a chromatogram for these compounds is illustrated here.** [Diagram explanation: The diagram shows a line titled "origin" on the left and "solvent front" on the right. Two spots, marked A and B, are placed along this line towards the origin, indicating two food colors.] **Which food color, A or B, would you predict to have the larger molecular weight?** Food Color A is having a larger molecular weight because food color that is close to the baseline is said to have more molecular weight. 6. **Give two real-life applications of chromatography.** 1. Food Testing 2. Drug Testing
# Report Sheet

### Sample Information:

| Sample        | Distance from origin to solvent front (cm) | Distance from origin to center of spot(s) (cm) | Rf (show calculations below the table) | Color of spot(s)  |
|---------------|--------------------------------------------|------------------------------------------------|---------------------------------------|-------------------|
| 1. Blue no. 2 | 7.2                                        | 1. 2.5                                         | 1. 0.35                               | 1. Red            |
|               |                                            | 2. 5.5                                         | 2. 0.76                               | 2. Blue-purple    |
| 2. Red no. 3  | 6.0                                        | 1. 1.2                                         | 1. 0.2                                | 1. Red            |
|               |                                            |                                                | 2.                                     |                   |
| 3. Yellow no. 5| 6.6                                       | 1. 0.7                                         | 1. 0.1                                | 1. Red            |
|               |                                            | 2. 3.0                                         | 2. 0.45                               | 2. Yellow         |
| Unknown No.   | 7.0                                        | 1. 0.13                                        | 1. 0.018                              | 1. Red            |
|               |                                            | 2. 3.2                                         | 2. 0.45                               | 2. Yellow         |
|               |                                            | 3. 1.5                                         | 3. 0.21                               | 3. Red            |

### Dyes in Unknown Mixture:
- Yellow (Rf = 0.45) and Red (Rf = 0.21)

### Calculations for Rf Values:
- 1. Blue no. 2
- 2. Red no. 3
- 3. Yellow no. 5
- Unknown No:

**Note:** Rf value is calculated as the distance from the origin to the center of the spot divided by the distance from the origin to the solvent front.
Transcribed Image Text:# Report Sheet ### Sample Information: | Sample | Distance from origin to solvent front (cm) | Distance from origin to center of spot(s) (cm) | Rf (show calculations below the table) | Color of spot(s) | |---------------|--------------------------------------------|------------------------------------------------|---------------------------------------|-------------------| | 1. Blue no. 2 | 7.2 | 1. 2.5 | 1. 0.35 | 1. Red | | | | 2. 5.5 | 2. 0.76 | 2. Blue-purple | | 2. Red no. 3 | 6.0 | 1. 1.2 | 1. 0.2 | 1. Red | | | | | 2. | | | 3. Yellow no. 5| 6.6 | 1. 0.7 | 1. 0.1 | 1. Red | | | | 2. 3.0 | 2. 0.45 | 2. Yellow | | Unknown No. | 7.0 | 1. 0.13 | 1. 0.018 | 1. Red | | | | 2. 3.2 | 2. 0.45 | 2. Yellow | | | | 3. 1.5 | 3. 0.21 | 3. Red | ### Dyes in Unknown Mixture: - Yellow (Rf = 0.45) and Red (Rf = 0.21) ### Calculations for Rf Values: - 1. Blue no. 2 - 2. Red no. 3 - 3. Yellow no. 5 - Unknown No: **Note:** Rf value is calculated as the distance from the origin to the center of the spot divided by the distance from the origin to the solvent front.
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