201_HW_4

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Mechanical Engineering

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Dec 6, 2023

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Christian Murrell BME 201 HW4 Q1. For the RNA sequence below indicate the amino acids that are encoded in the three reading frames. If you were told that this segment of RNA was in the middle of an mRNA that encoded a large protein, would you know which reading frame was used? How so? AGUCUAGGCACUGA Reading Frame 1: Ser-Leu-Gly-Thr Reading Frame 2: Val-STOP Reading Frame 3: Ser-Arg-His-STOP If this segment was in the middle of an mRNA, it would be reading frame 1 since frame 1 does not have a stop codon. Q2. Which of the following mutational changes would you predict to be the most deleterious to gene function? Explain your answer. a) Insertion of a single nucleotide near the end of the coding sequence b) Removal of a single consecutive nucleotide near the beginning of the coding sequence c) Deletion of three consecutive nucleotides in the middle of the coding sequence d) Deletion of four consecutive nucleotides in the middle of the coding sequence e) Substituting of one nucleotide for another in the middle of the coding sequence
Removal of a single consecutive nucleotide near the beginning of the coding sequence would be the most deleterious option. Removing a nucleotide would shift the reference frame resulting in a completely different mRNA translation. Additionally, since this removal would occur at the beginning of the sequence, majority of the mRNA sequence will be translate the wrong amino acids. Q3. One strand of a section of DNA isolated from E. coli reads 5’-GTAGCCTACCCATAGG- 3’ (1) Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will be the sequence of the mRNA in this region? The sequence would be CAUCGGAUGGGUAUCC (2) How many different peptides could be potentially made from this sequence of RNA? 4 different peptides could be made from the sequence of RNA. An amino acid polypeptide could be made if it began at the start codon. Also, a 5 amino acid polypeptide could form if the start codon appeared before the sequence of the first frame. Lastly, 2 polypeptides could be made by shifting the reading frame to the 2 nd and 3 rd . (3) Would the same peptides be made if the other strand of the DNA served as the template for the transcription? No, the same peptides would not be made if DNA was used instead of mRNA. It would form completely different amino acids since there are a different set of nucleotides in DNA than that of mRNA.
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