Introduction to Histology_Reproductive system answer sheet

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Feb 20, 2024

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Introduction to Histology: Reproductive System answer sheet Observations Take a picture and insert it in the appropriate section below also record the total magnification needed to best view the object. Label all slides as indicated in the Lab Manual. Testis Magnification used: 100x SEPTA SPERMATOZOA SEMINIFEROUS TUBULE INTERSTITIAL CELL BLOOD VESSEL

©2015 Carolina Biological Supply Company 3 Mammal Ovary Follicles Magnification used: 100x Human Uterus Magnification used: 100x ATRUM PRIMARY FOLLICLE SECONDARY FOLLICLE CORPUS LUTEUM UTERINE GLAND LUMEN ENDOMETRIUM

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©2015 Carolina Biological Supply Company 4 Data table Fill out the following table based on the information listed in the lab manual. Tissue type Testis Best magnification Provide at least 2 unique identifying features of this tissue type Describe how these tissues appear under the microscope Septa 400x Fibrous partitions, dense connective tissue, loose areolar Thick purple (stained) tissue, close to the edge Interstitial cells 400x Surrounded by blood vessels, cells Thick cells formed closely together Spermatozoa 400x Lined with epithelial cells, tubules Spaced out tissues, purple (stained) with spots Seminiferous tubule 400x Lined with epithelial cells, found sertoli cells Divided, contained within the lobules, column shaped Blood vessel 400x Contained within the tunica albuginea, tubular shaped, vessels all have openings Solid, tube shaped lines

©2015 Carolina Biological Supply Company 5 Tissue type Ovary Best magnification Provide at least 2 unique identifying features of this tissue type Describe how these tissues appear under the microscope Oocyte nuclei 100x Large nucleus, germ cells No outer layer on the nucleus Follicle 100x Grainy rounded layer, small sacs Graining and rounded, appear to contain hair Antrum 100x Large space containing follicular fluid, pyloric canal Large open space (pyloric atrium) Primary oocyte 100x Large nucleus, germ cells, follicles Large round nucleus, visible follicles Granulosa cells 100x Cells surrounding the oocyte, follicles Round and grainy in appearance, visible follicles Tissue type Follicles Best magnification Provide at least 2 unique identifying features of this tissue type Describe how these tissues appear under the microscope Primary follicle 100x Granulosa surrounding the oocyte, has openings Cells divide and surround the oocyte, multiple openings Secondary follicle 100x Cells divide, enlarged intracellular spaces, consists of oocytes Larger than the primary follicle, oocytes present

©2015 Carolina Biological Supply Company 6 Questions 1. What is a granulosa cell? What is characteristic of a granulosa cell under the microscope? What is the function of the granulosa cell? The granulosa cell or follicular cell is a somatic cell of the sex chord that is closely associated with the developing female gamete (oocyte of egg) in the ovary. Granulosa cells form a single layer around the female gamete in the primordial Tertiary follicle 100x Also referred vesicular follicle, fluid filled follicle, granulose epithelial cells Larger than the secondary follicle, epithelial cells Corpus luteum 100x Formed by granulose cells, Tertiary follicles collapse and slowly degenerate Empty space where the oocyte is deposited, lutein cells Tissue type Uterus Best magnification Provide at least 2 unique identifying features of this tissue type Describe how these tissues appear under the microscope Myometrium 100x Three layers of smooth muscle cells, fundus thick muscle layer, middle circular muscular cells, Endometrium 100x Simple columnar epithelial cells, glands Simple purple (stained) columnar epithelial tissue Uterine gland 100x Between columnar epithelial cells, visible blood vessels, smooth muscle Tall single layer of cells, columnar ciliated Columnar epithelium 100x Column shaped epithelial cells, thick parts Tall single layer of cells, multiple nuclei

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©2015 Carolina Biological Supply Company 7 ovarian follicle. The function of the granulosa cells is the production of steroids and LH receptors to further enlarge the oocyte for the further follicle development. The granulosa cells will also secrete progesterone after ovulation. 2. What are the differences in appearance on a slide between the endometrium and myometrium? What is the physiological reason for these differences? Endometrium is the inner glandular layer of the uterus wall comprised of simple columnar epithelial tissue whereas myometrium is the middle muscular layer of the uterus wall comprised of three layers of smooth muscle cells. The myometrium (middle layer) conducts contractions of the uterus during delivery, child birth, and parturition whereas the endometrium (inner layer) undergoes uterus cyclic changes during menstruation and embryo implantation. 3. How can we easily distinguish the spermatozoa in a slide of the testis? Why do they look the way that they do The spermatozoa are deep purple in color with a visible head, neck, and tail located close to the tubule. The spermatozoa have a streamlined body to allow it to move rapidly to reach the target egg cell. For instance, the head has a tapering apex (top) which limits the amount of resistance incurred while traveling within the female reproductive system. 4. What is different between a primary follicle and secondary follicle in a microscope slide? Why? The primary follicle is surrounded by single layer of granulosa cells surrounding the oocyte whereas the secondary follicle are larger and consists of many layers of membrana granulosa cells surrounding the oocyte. The secondary follicle also contains small accumulations of fluid in the intracellular spaces (follicular fluid). 5. What tissue type is very abundant in the tunica albuginea? The type of tissues that is very abundant in the tunica albuginea is connective tissue, especially elastin and collagen.

Introduction to Histology_Reproductive system answer sheet

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