Proximate Analysis Assignment Key

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University of Wisconsin, River Falls *

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213

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Health Science

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Feb 20, 2024

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- Name; /< E Y Lab Section: 12L 13L 14L Animal Scielice>231 Spring 2024 Proximate Analysis Assignment Use 2 decimal places throughout To make calculations easier, please use the conversion of 1 kg = 2.2 Ib when needed Abbreviations to know: DM = dry matter, OM = organic matter, EE = ether extract Oralf) CP = crude protein, CF = crude fiber REVIEW: Use this section to work on you own. Fill in the table below for a swine diet going to the feed mill. Feed Ingredient % of diet kg Ib Corn grain 2;}’22;7 )W&? =l g 2,300 ¥ 2.2 £, 0000 Soybean meal 24,00 800 I, 700 Choice grease 2.6 95 20 ? Supplements 2.77 _ 125 Ly Total 100% 3,320 kg 7 30 v **4lways check your totals** Fill in the table below with the crude protein concentration of the feedstuff on either an as-fed or DM basis as indicated. Crude protein, % Feedstuff DM, % As-fed DM basis ~ Calculations basis 1 ©91.00 17.00 /5),(0(9 /7/0‘ G) 2 30.00 r AR, 12.00 12.% 0:.20 Fill in the table below with the INTAKE of the feedstuff on either an as-fed or DM basis as indi;:atéd. Intake, 1b Feedstuff DM, % As-fed DM basis Calculations basis 1 75.00 15.50 //@3 i s X 075 2 91.50 4.6 4.50 Y, {0/0'4/5—
- NEW MATERIAL 1. In the table below, classify each nutrient as either: vitamin, mineral, amino acid, or fatty acid. Nutrient Classification: vitamin, mineral, amino acid, fatty acid Arachidonic acid ]/ i 5 ) oty O Ir s N o YW\ vron Pantothenic acid Vi n Cobalamin : , Vi+o o v Alanine (/’ 14 1 2aY14W) &Cl g Leucine %}VV\‘I(\D (;LC,JCJA Glutamic acid Linoleic acid B inne QQJA Eatty ocid Zinc irdrond Thiamin Vo rin 2. Watch the Proximate Analysis Percent Ash video to answer the following questions. https://www.youtube.com/watch?v=25eqoDnvoJA a. For animal feeds and grains: Muffle furnace temperature = Ashing time (hold time) = Z b0 -c hours b. It is good practice to stack the crucibles on top of one another in the muffle furnace. True False c. Using the lab data below, calculate percent ash and organic matter. Empty crucible | Sample weight Weight of Ash weight | % Ash % Organic weight (grams) | (grams) crucible and (grams) matter ash (grams) 46.25 1.98 46.38 013 lo. $7 (/\g L/g Ylp. 38 46 25 013 -6 S 7 'L9&>*JOO /OO 3. Watch the Proximate Analysis Percent Protein video to answer the following questions. https://www.youtube.com/watch?v=K87FQf yYYE a. Which method is being demonstrated in the video: ( LECO/Combustion b. What is the combustion gas used: Oy (/J Sl Kjeldahl ol opn c. What is the carrier gas used: EDTA d. What is the internal standard used:
e. Generally, most samples use a 6.25 factor for calculation of protein. A different factor may be warranted when very high-quality proteins are being determined such as dairy products. What is the suggested factor to use when analyzing dairy products? (0. 2 8 f. Below are your % nitrogen values from the lab. Calculate % protein in the feed samples. Nitrogen, % X lo. 25 Crude protein, % 7:9| /S, T Feed sample A 125 B 2,58 4. Watch the Proximate Analysis Percent Fat video to answer the following questions. https://www.youtube.com/watch?v=ylyjm8i¥Y23Q a. Which two organic solvents are typically used for this analysis? Po i voleum Hexanrnd b. Calculate the percent fat from the following lab data. ' Empty cup weight | Sample weight Ending cup weight | Fat weight % Fat (grams) (grams) (grams) (grams) . 50.50 6.50 52.45 4 /75 20 (52.95-50.50D 7"%5/.<0) K 100 5. Nitrogen free extract (NFE) is a calculated estimate of the digestible carbohydrates (starches, sugars, and carbohydrates that ARE NOT fiber) in a feedstuff or diet. It is not analyzed using a laboratory method but is calculated using the following equations: ® NFE (As-fed basis) = 100 moisture CP EE CF ash (using the as fed values of components) ® NFE (DM basis) = 100 CP EE CF - ash (using the dry matter values of components) g 4 o T - sl ) = (P‘{ Based on the data in the table, calculate NFE. (00~ 35~ /2 - %52~ (8 ~/ 7C 2 Sample Moisture, % | CP, % as fed | EE, % asfed | CF, % as fed | Ash, % asfed | NFE, % as fed A 35.00 12.00 4.52 6.78 1.70 247 B 10.00 18.00 1.75 13.25 2.67 sy 33 Based on the data in the table, calculate NFE. jpp - 2.8 /&~ 4.4 - 5.5 = &7 Sample % DM CP, % DM EE, % DM CF, % DM Ash, % DM NFE, % DM A 75.00 28.00 15.00 4.50 5.50 7 B 90.00 12.00 4.00 10.45 2.50 08
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