Care and use of a Pipette

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Texas State University *

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MISC

Subject

Chemistry

Date

Feb 20, 2024

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docx

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3

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Care and use of a Pipette Abstract This experiment is to help understand the use of a pipette. As in this lab we will continue to use pipettes to be able to transfer specific sample fluids. This is important because there could be several factors to be able to maintain an accurate volume transfer. The main idea of this lab was to see the error that could be made when using a pipette and also the difference of volume in size. The result in the lab showed that there could be errors made while pipetting. Using a pipette is understood more now because there was a specific way to use a pipette to make sure the right amount of liquid is being removed. Introduction In this lab we conducted two experiments which both will test the accuracy of pipetting. We will be understanding the use of a pipette and the factors that come in place when using it. A pipette is used to transfer a specific amount of volume. There is a specific technique when using a pipette if not done correctly there could be an error with the volume consumed. This lab also will show how to properly show how to change the different volumes to be able to get the right amount. Methods and Material The materials in this lab are a pipette ( P20, P200,P1000), a beaker which contains our fluid, balance, white filter paper, Laemmli Buffer and centrifuge tube. First we collect our materials and make sure the pipettes are set to the correct volume, we will then weigh a 15ml centrifuge tube on the balance with the cap removed. Then we will place the pipette in the fluid, where we will remove the fluid from the beaker and transfer it to the centrifuge tube. Then we will weigh the centrifuge with the fluid collected. We will be doing this with 4 different volumes which are P20 (20ul), P200(30ul), P200 (180ul) and P1000 (250ul), and P1000(850ul). Each volume we will do three different trials. Then we will calculate the %error and avg error. For the second experiment we used a Laemmli buffer and filter paper. We will then set our P-20 pipette to 2ul and place 5 different dots lined up then do another set of 5 dots lined up next to them but change the pipette to 3ul. Results The result of the experiment was to show how close we could get to the expected weight and to calculate what our percent error was. At the end we avg out the error of our three trials and the highest average error we got was 96.6% for the P200 and the volume was 30 ul. This avg percent shows that something went wrong during the experiment. Which probably was not using the pipette properly.
P20 Volume Expected Actual % error Average error 1 20 ul 0.02 g 0.01 50% 33% 2 20 ul 0.02 g 0.01 50% 33% 3 20ul 0.02 g 0.02 0% 33% P200 1 30 ul 0.03 g 0.03 0% 96.6% 2 30 ul 0.03 g 0.07 130% 96.6% 3 30 ul 0.03 g 0.08 160% 96.6% P200 1 180 ul 0.180 g 0.18 0% 5% 2 180 ul 0.180 g 0.19 5% 5% 3 180 ul 0.180 g 0.20 10% 5% P1000 1 250 ul 0.25 g 0.23 8% 6.6% 2 250 ul 0.25 g 0.23 8% 6.6% 3 250 ul 0.25 g 0.24 4% 6.6% P1000 1 850 ul 0.850 g 0.94 10.59% 9.8% 2 850 ul 0.850 g 0.93 9.41% 9.8% 3 850 ul 0.850 g 0.93 9.41% 9.8%
Discussion The results in this lab were to show how much of an error was made when transferring the fluid into the centrifuge. After taking the % error we calculated the average error where we only got one high average error out of all the other trials. While the highest average error was 96.6% this indicates that something went wrong during the transferring of fluid which maybe the volume was set wrong, or the technique of collecting the fluid was done wrong. Doing this experiment helps better understand how to use a pipette. There is a specific way to grab the fluid and if the technique is done wrong you could be grabbing more fluid then expected. Conclusion The experiment itself was correctly understood and it was done to teach us the correct technique. The technique of using a pipette should be practiced as much as possible till perfected because it will be continued to be used throughout the lab and error should be limited.
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