CH250 Experiment 1_ Fundamentals of Aqueous Biochemistry · Benchling
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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●
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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●
Solution F
pH= ±±.²³
Solution G
pH= ±.²²
Solution H
pH= ±.³´
Solution I
pH= ±.µ¶
Solution J
pH= ´·.¸²
A
B
C
1
2
3
4
5
pH of solutions F-J with 0.1 M of NaOH (…
Solution A
pH= ¶.±¸
Solution B
pH= ±.±±
Solution C
pH= ±.¹µ
Solution D
pH= ±.´·
Solution E
pH= ¶.º·
Solutions
pH
1
2
3
4
5
pH of solutions A-E with 0.1 M of HCl …
1/17/22, 4:24 PM
Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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Solution B
´/ (±.²·-±.±±) = ´³.³³
Solution C
´/ (±.²·-±.¹µ) = µ.¹¹
Solution D
´/ (±.²·-±.´·) = ¶.²
Solution E
´/ (±.²·-¶.º·) = ·.²²
Solution G
´/ (±.²·-±.²²) = -¶·
Solution H
´/ (±.²·-±.³´) = -¸.·¸
Solution I
´/ (±.²·-±.µ¶) = -¹.´¶²
Solution J
´/ (±.²·-´·.¸²) = -·.´²²
Solutions
Buffer
Capacity
1
2
3
4
5
6
7
8
Buffer Capacity
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
https://benchling.com/ish_sharma28/f/lib_fjGB0zxA-ch250-ishika-sharma-lab-4/etr_9BzEBA5n-experiment-1-fundamentals-of-aqueous-biochemistry/view?versionI…
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A
Cannot be calculated
B
·.·¹¹ mol/mL
C
·.·³³ mol/mL
D
·.¶ mol/mL
E
´ mol/mL
F
Cannot be calculated
G
·.·¹¹ mol/mL
H
·.·³³ mol/mL
I
·.¶ mol/mL
J
´ mol/mL
Solution
Final
Concentration
1
2
3
4
5
6
7
8
9
10
Table1
1/17/22, 4:24 PM
Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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Experiment 1: Fundamentals of Aqueous Biochemistry · Benchling
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What is the main difference between a qualitative analysis and a quantitative
analysis of a solution?
The two must be done independently of each other, as they are completely
unlike.
A qualitative analysis must always be done before a quantitative analysis.
A quantitative analysis must always be done before a qualitative analysis.
A qualitative analysis identifies the type of ion in solution and the quantitative
analysis will determine how much of this ion is present.
A quantitative analysis identifies the type of ion in solution and the qualitative
analysis will determine how much of this ion is present.
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O THERMOCHEMISTRY
Griffin
Calculating a molar heat of reaction from formation enthalpies
Using the table of standard formation enthalpies that you'll find under the ALEKS Data tab, calculate the reaction enthalpy of this reaction under standard
conditions:
2C,H;(g)+70,(9)→4 CO,(g)+6H,O()
Round your answer to the nearest kJ.
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isms
ots 2req
Use the References to access important values if needed for this question.
In a study of the conversion of methyl isonitrile to acetonitrile in the gas
phase at 250 °C
Visited
1 pts 2req
CH3NC(g)-
>CH3CN(g)
1 pts 2req
the concentration of CH NC was followed as a function of time.
It was found that a graph of In[CH3NC] versus time in seconds gave a
1 pts 2req
straight line with a slope of -3.96x10-3 s and a y-intercept
52.
1 pts 2req
Based on this plot, the reaction is
v order in CH,NC and the
rate constant for the reaction is
1 pts 2reg
1 pts 2reg
1 pts 2reg
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1. If the concentration of solute in a solution is high, the Absorbance will
be high or low and why?
2. Was the spectroscopy experiment qualitative or quantitative analysis?
3. If we have 12M HCI and we need 3 L of 1M HCI, how much 12 M
should we use?
4. What does Molarity mean?
5. When must safety goggles be worn?
6. If you make a mistake recording data, what must you do?
spec hw.pptx (2/3)
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2. A linear standard curve of KMnO4 is prepared from a set of standard solutions by plotting
absorbance (y) vs. concentration (x). Use Logger Pro (as described in the Data Analysis for this
experiment), or Google Sheets/Excel, to plot this standard curve using the data in Table 1. Help with
spreadsheets [PDF]
Table 1: Data for the standard curve of KMnO4
Absorbance Concentration (M)
0.372
6.00 x 10-4
0.298
0.223
0.149
0.0744
After plotting the standard curve, enter its slope below (not the equation of the line). Report your
answer to 3 significant figures. If your answer ends in one or more zeros, use scientific notation.
Slope =
4.80 x 10-4
3.60 x 10-4
2.40 x 10-4
1.20 x 10-4
M-1
M
3. Suppose that an unknown sample is analyzed spectrophotometrically at the same wavelength that
was used for the standard curve and the absorbance was found to be 0.186. Use the standard curve
to find the concentration of the unknown. Hint:
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Chapter 8b: EOC (sec 4-7)
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O 1 pt
Determine the partial pressure of each gas as shown in the figure below. Note: The relative numbers of each type of gas are depicted in the figure.
Question 1
Question 2
1 pt
1 atm
Question 3
1 pt
o He
Question 4
1 pt
O Ne
Ar
Question 5
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Question 6
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Partial pressure of He =
atm
Partial pressure of Ne-
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Partial pressure of Ar =
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Give the nuclear symbol (isotope symbol) for the isotope of bromine, Br, that contains 46 neutrons per atom. Replace the
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ge 2: Problems of Water and Air Quality Questions
1. Why is it recommended to have a carbon monoxide detector on each floor of a home?
Et
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caused by acid rain?
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The COVID-19 pandemic forced many people to work from home for extended periods. During
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The following equation describes the dependence of the rate constant (k) with the temperature in dynamic
NMR.
kgTc
exp (-
-AG*
RTc
k =
h
where kg and h are physical constants, R is the ideal gas constant, T. is the coalescence temperature and
AG* is the activation energy? The following table shows the units of some of these parameters.
Parameters
Units
k
s-l
T.
K
h
Js
J K-' mol-
J mol-
R
AG+
What are the units of kg? Show your analysis.
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Referonces
Use the References to access important values if needed for this question.
For the following reaction, 50.4 grams of sulfur dioxide are allowed to react with 17.9 grams of water.
sulfur dioxide (g) + water (I) sulfurous acid (H2SO3) (g)
grams
What is the maximum amount of sulfurous acid (H,SO3) that can be formed?
What is the FORMULA for the limiting reagent?
grams
What amount of the excess reagent remains after the reaction is complete?
Submit Answer
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The following equation describes the dependence of the rate constant (k) with the temperature in dynamic
NMR.
kgTc
-AG*.
exp (-
RT.
k =
C
where kg and h are physical constants, R is the ideal gas constant, T. is the coalescence temperature and
AG* is the activation energy? The following table shows the units of some of these parameters.
Parameters
Units
k
s-1
To
K
h
Js
R
JK-' mol-!
AG*
J mol-
Does k increase with AG*? Explain your answer.
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H
CH3
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2)
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OCH3
TiCl4
CH₂Cl₂/-78°C / 15 min
PPh3/1₂ (1 eq)
imidazole / THE
0°C-it
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caffeine
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1
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69.58
How to get the concentration of the mixture in this case?
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4 and 15
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This question has multiple parts. Work all the parts to get the most points.
1 pt
2 pts
Data in the table were collected at 540 K for the following reaction:
2 pts
CO(g) + NO2(g) → CO2 (g) + NO(g)
1 pt
Initial concentration
(mol/L)
Initial rate
2 pts
[CO]
[NO2]
(mol/L. h)
1 pt
5.1 × 10-4
0.35 × 10
3.9 × 10-8
2 pts
1pt
5.1 × 10-4
0.70 × 10-4
7.9 × 10-8
0
2 pts
5.1 x 10-
0.18 × 10-4
2.0 × 10-8
1
1pt
1.0 × 10-3
0.35 × 10-4
7.7 x 10-8
2
1 pt
1.5 × 10-3
0.35 × 10-4 1.2 × 10-7
3
1 pt
4
1 pt
5
1 pt
a Determine the reaction order with respect to each reactant.
Order with respect to CO: 1
Order with respect to NO2:
Submit
ns
28 at
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5. Calculate AH for the reaction
N2H4 (1) + O2 (g) - N2 (g) + 2 H20 (I)
given the following data:
2 NH3(g) + 3 N;O(g) ----> 4 N2(g) + 3 H2O(1) AH° = -1010. kJ
N2O(g) + 3 H2(g) ----> N2H¾(1) + H2O(I)
AH° = -317 kJ
2 NH3(g) + 1/2 O2(g) ----> N2H4(1) + H¿O(1) AH° = -143 kJ
H2(g) + 1/2 O2(g) ----> H2O(1)
AH° = -286 k)
** Be sure to show all of your manipulated equations.
rr
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You obtained the following raw data when setting up a Bradford standard curve:
BSA (mg/ml)
Absorbancy 595nm
0
0.225
1
0.310
2
0.420
3
0.510
4
0.610
5
0.720
6
0.810
7
0.915
8
0.950
9
0.980
10
0.990
After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained.
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
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One way the U.S. Environmental Protection Agency (EPA) tests for chloride contaminants in water is by titrating a sample of silver nitrate solution. Any chloride
anions in solution will combine with the silver cations to produce bright white silver chloride precipitate.
Suppose an EPA chemist tests a 200. mL sample of groundwater known to be contaminated with iron(II) chloride, which would react with silver nitrate solution
like this:
FeCl,(aq) + 2 AgNO3(aq) → 2 AgCl(5) + Fe(NO3),(aq)
2
The chemist adds 11.0 mM silver nitrate solution to the sample until silver chloride stops forming. She then washes, dries, and weighs the precipitate. She finds
she has collected 8.9 mg of silver chloride.
ol.
Calculate the concentration of…
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a) Blank 1
b) Blank 2
c) Blank 3
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Good Day
03 Be O +2 AIC→I AbO, •3 Be Cla
a) what is the molar mass? BeO , AlzOa
b) How many mols of beryllium chleride are produced from
13 mols of BeO?(1-step)
) How many grams of Al,O, are
9 mol AICI,? (2-step)
) How many grams of Al2Og are produced Srom
100g BeO (3-step)
e) Determine the limit ing reartan ti 13 7mol BeO ;13.7mol Al Cl,
produced from
5) Determine excess reactant 4Sa BrO j 75q AICIS
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HW 4.4
Milk of magnesia, which is an aqueous suspension of magnesium hydroxide, is used as an
antacid in the reaction below. How many molecules of HCI would have to be present to form
17.38 g of MgCl?
7
Mg(OH)2 (s)
2 HCI (aq)2 H2O (I)MgCla (aq)
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MEASUREMENT AND MATTER
Setting up the math for a one-step problem with unit conversion
The speed of light is 2.998 x 108 m/s. How far does light travel in 3.0 µs?
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Also, be sure your answer includes all the correct unit symbols.
distance =
x10
0.0
X
F
00
4
O
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Round your answer to zero decimal places.
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Publisher:Cengage Learning
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Publisher:McGraw-Hill Education
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Chemistry: Principles and Reactions
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ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning
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Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:WILEY
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