01 WS Chromatography
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University of Houston, Clear Lake *
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1112
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Chemistry
Date
Feb 20, 2024
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docx
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CHEM1112-
section#
Experiment #1
Your Name
Date
Chromatography
Lab Partner: Lab Partner’s Name
Procedure (3 pts)
During this experiment it was decided to start with the second part since the first part would take less time. First the students had to get the color off the candy to do that add a drop or two or water and scape it off the candy without getting the chocolate. Next add a drop of the color from the candy to the chromatography paper as well as the corresponding FD+C color. After that add the paper to a beaker that has
NaCl covering the bottom and wait for the liquid to travel across the paper. After that was set up, we started the next part. 3 different isopropanol solutions were used for this part 5%, 28% and 70%.to get out the red of the purple Kool-Aid we used 5% isopropanol next a 28% solution was used that gave us a blue tint and to make sure all the color
was out of the column we used a 70% solution. After that the results were recorded. We collected the Rf values from the paper chromatography after the NaCl solution reached the top of the paper. Data and Observations (12 pts)
Part I. Kool-Aid Separation
Fraction
Visual Characteristics
Other Characteristics
5%
Red tint
Faint stawberry scent
28%
Blue tint
Grape scent
70%
clear
Part II. Paper Chromatography on Candy or Sharpies
CHEM1112-
section#
Experiment #1
Your Name
Date
Chromatography
Lab Partner: Lab Partner’s Name
Blended together.
Solvent traveled:11.3 cm
Red-2cm Rf- 0.18
FD+C Red- 8.5 cm Rf- 0.75
Yellow-5.7 cm Rf- 0.5
FD+C Yellow- 5.7 cm Rf- 0.5
Potential Error Sources
During the experiment we had our paper chromatography merge together this could have given us false values for when we calculated our Rf values. Another source of error could be that we didn’t clean our tools correctly and had cross contamination.
Postlab Questions (5 pts)
1.
[Part I] Describe how the solvents worked as the mobile phase of the liquid chromatography
experiment. Why was it necessary to use different concentrations of aqueous isopropanol in the
step-gradient separation? Yes, it was necessary because the different gradients took out different things
from the solution.
2.
[Part I] How can one improve separation of compounds using column chromatography? You can
improve the efficiency to make it faster.
3.
[Part II] Some people are allergic to the dye Yellow #5 (tartrazine). Should they be concerned
about any of the candy? Yes skittles and M&M’s both have yellow #5.
4.
[Part II] The candy coatings contain a variety of other ingredients (sugars, binders, gums, etc.)
that also have the potential of dissolving in water. Can these alter the R
f
values from the values of the
pure dyes in the solvent? Yes, because they could have lightened the color to make it not go as far.
5.
[Part II] Propose an experiment how chromatography could be used to check purity or identity
of a drug tablet/food item. You could use thin layer chromatography to do this experiment.
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NaCl
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